Talk:IGEM:MIT/2006/Communications: Difference between revisions
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*Funding sources for each person | *Funding sources for each person | ||
*Protocol for transformation | *Protocol for transformation | ||
*Describing figure 6 | *Describing figure 6 | ||
*Update <bbpart>BBa_Q04401</bbpart> | *Update <bbpart>BBa_Q04401</bbpart> | ||
Revision as of 09:43, 11 February 2008
Questions for Stephen
- Did you use the Knight or Endy site directed mutagenesis protocol? (http://openwetware.org/wiki/IGEM:MIT/2006/Notebook/2006-8-10#Mutagenesis says Knight but you told me Endy)
- A: We used the Knight protocol.
- Then are you sure that you phosphorylated the primers? The Knight protocol doesn't call for this step.
- A: We used the Knight protocol.
- Do we have the regulated wintergreen GC data in a form that we could generate plots similar to the banana timecourse?
- What did you use to shake the cultures? i.e. what incubator or shaker?
General outstanding issues
- Funding sources for each person
- Protocol for transformation
- Describing figure 6
- Update <bbpart>BBa_Q04401</bbpart>
