Talk:IGEM:MIT/2006/Communications

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(Questions for Stephen)
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#*A: We used the Knight protocol.
#*A: We used the Knight protocol.
#**Then are you sure that you phosphorylated the primers?  The Knight protocol doesn't call for this step.
#**Then are you sure that you phosphorylated the primers?  The Knight protocol doesn't call for this step.
 +
*I'm sure we did.
#Do we have the regulated wintergreen GC data in a form that we could generate plots similar to the banana timecourse?
#Do we have the regulated wintergreen GC data in a form that we could generate plots similar to the banana timecourse?
 +
*Yes, we could.  It would take some digging through old files and sorting out through each protocol we used on each day.  I don't know if it's necessary to show though.
#What did you use to shake the cultures?  i.e. what incubator or shaker?
#What did you use to shake the cultures?  i.e. what incubator or shaker?
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 +
220 RPM- the shaker in 37 C room
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110 RPM- the shaker by Samantha's/2007 iGEM team's bench
==General outstanding issues==
==General outstanding issues==

Revision as of 13:46, 11 February 2008


Questions for Stephen

  1. Did you use the Knight or Endy site directed mutagenesis protocol? (http://openwetware.org/wiki/IGEM:MIT/2006/Notebook/2006-8-10#Mutagenesis says Knight but you told me Endy)
    • A: We used the Knight protocol.
      • Then are you sure that you phosphorylated the primers? The Knight protocol doesn't call for this step.
  • I'm sure we did.
  1. Do we have the regulated wintergreen GC data in a form that we could generate plots similar to the banana timecourse?
  • Yes, we could. It would take some digging through old files and sorting out through each protocol we used on each day. I don't know if it's necessary to show though.
  1. What did you use to shake the cultures? i.e. what incubator or shaker?

220 RPM- the shaker in 37 C room

110 RPM- the shaker by Samantha's/2007 iGEM team's bench

General outstanding issues

  • Funding sources for each person
  • Protocol for transformation
  • Describing figure 6
  • Update <bbpart>BBa_Q04401</bbpart>
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