Talk:IGEM:MIT/2006/Communications: Difference between revisions
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#*A: We used the Knight protocol. | #*A: We used the Knight protocol. | ||
#**Then are you sure that you phosphorylated the primers? The Knight protocol doesn't call for this step. | #**Then are you sure that you phosphorylated the primers? The Knight protocol doesn't call for this step. | ||
*I'm sure we did. | |||
#Do we have the regulated wintergreen GC data in a form that we could generate plots similar to the banana timecourse? | #Do we have the regulated wintergreen GC data in a form that we could generate plots similar to the banana timecourse? | ||
*Yes, we could. It would take some digging through old files and sorting out through each protocol we used on each day. I don't know if it's necessary to show though. | |||
#What did you use to shake the cultures? i.e. what incubator or shaker? | #What did you use to shake the cultures? i.e. what incubator or shaker? | ||
220 RPM- the shaker in 37 C room | |||
110 RPM- the shaker by Samantha's/2007 iGEM team's bench | |||
==General outstanding issues== | ==General outstanding issues== | ||
Revision as of 10:46, 11 February 2008
Questions for Stephen
- Did you use the Knight or Endy site directed mutagenesis protocol? (http://openwetware.org/wiki/IGEM:MIT/2006/Notebook/2006-8-10#Mutagenesis says Knight but you told me Endy)
- A: We used the Knight protocol.
- Then are you sure that you phosphorylated the primers? The Knight protocol doesn't call for this step.
- A: We used the Knight protocol.
- I'm sure we did.
- Do we have the regulated wintergreen GC data in a form that we could generate plots similar to the banana timecourse?
- Yes, we could. It would take some digging through old files and sorting out through each protocol we used on each day. I don't know if it's necessary to show though.
- What did you use to shake the cultures? i.e. what incubator or shaker?
220 RPM- the shaker in 37 C room
110 RPM- the shaker by Samantha's/2007 iGEM team's bench
General outstanding issues
- Funding sources for each person
- Protocol for transformation
- Describing figure 6
- Update <bbpart>BBa_Q04401</bbpart>
