Talk:IGEM:MIT/2006/Communications: Difference between revisions

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#*110 RPM- the shaker by Samantha's/2007 iGEM team's bench
#*110 RPM- the shaker by Samantha's/2007 iGEM team's bench
#Are both J45200 and J45250 on pSB1AT3?
#Are both J45200 and J45250 on pSB1AT3?
A: Yes.
#What concentration salicylic acid and isoamyl alcohol did we use for the scent generator tests?  Was it always 440 ppm isoamyl alcohol?
#What concentration salicylic acid and isoamyl alcohol did we use for the scent generator tests?  Was it always 440 ppm isoamyl alcohol?
A: Always 440 PPM isoamyl alcohol, 276 PPM salicylic acid
#A summary of your analysis of fixing the wintergreen precursor production would be useful
#A summary of your analysis of fixing the wintergreen precursor production would be useful


==General outstanding issues==
==General outstanding issues==
*Update <bbpart>BBa_Q04401</bbpart> (Jason)
*Update <bbpart>BBa_Q04401</bbpart> (Jason)

Revision as of 12:18, 16 February 2008


Questions for Stephen

  1. Do we have the regulated wintergreen GC data in a form that we could generate plots similar to the banana timecourse?
    • Yes, we could. It would take some digging through old files and sorting out through each protocol we used on each day. I don't know if it's necessary to show though.
      • Drew would like to show these data if we can.
  2. What did you use to shake the cultures? i.e. what incubator or shaker?
    • 220 RPM- the shaker in 37 C room
    • 110 RPM- the shaker by Samantha's/2007 iGEM team's bench
  3. Are both J45200 and J45250 on pSB1AT3?

A: Yes.

  1. What concentration salicylic acid and isoamyl alcohol did we use for the scent generator tests? Was it always 440 ppm isoamyl alcohol?

A: Always 440 PPM isoamyl alcohol, 276 PPM salicylic acid

  1. A summary of your analysis of fixing the wintergreen precursor production would be useful

General outstanding issues

  • Update <bbpart>BBa_Q04401</bbpart> (Jason)