Talk:IGEM:MIT/2006/Communications

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Questions for Stephen

  1. Did you use the Knight or Endy site directed mutagenesis protocol? (http://openwetware.org/wiki/IGEM:MIT/2006/Notebook/2006-8-10#Mutagenesis says Knight but you told me Endy)
    • A: We used the Knight protocol.
      • Then are you sure that you phosphorylated the primers? The Knight protocol doesn't call for this step.
  2. Quantification of banana timecourse
    • A: I sent both Alex and Amy an email today about a meeting tomorrow.
      • You might want to do some background research on quantification of GC data.
  3. Does <bbpart>BBa_J45017</bbpart> still have the PstI site or has it been mutated out? If it has been mutated out, then the Registry needs to be updated.
  4. Update the sequence of <bbpart>BBa_J45009</bbpart> to reflect the fact that BioBrick sites weren't eliminated.
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