Talk:IGEM:MIT/2006/Communications
From OpenWetWare
Jump to navigationJump to search
Questions for Stephen
- Did you use the Knight or Endy site directed mutagenesis protocol? (http://openwetware.org/wiki/IGEM:MIT/2006/Notebook/2006-8-10#Mutagenesis says Knight but you told me Endy)
- A: We used the Knight protocol.
- Then are you sure that you phosphorylated the primers? The Knight protocol doesn't call for this step.
- A: We used the Knight protocol.
- Quantification of banana timecourse
- A: I sent both Alex and Amy an email today about a meeting tomorrow.
- You might want to do some background research on quantification of GC data.
- A: I sent both Alex and Amy an email today about a meeting tomorrow.
- Does <bbpart>BBa_J45017</bbpart> still have the PstI site or has it been mutated out? If it has been mutated out, then the Registry needs to be updated.