Talk:IGEM:MIT/2006/Communications

• Author guidlines for Nature MSB
• PNAS information for authors

Questions for Stephen

1. Did you use the Knight or Endy site directed mutagenesis protocol? (http://openwetware.org/wiki/IGEM:MIT/2006/Notebook/2006-8-10#Mutagenesis says Knight but you told me Endy)
   • A: We used the Knight protocol.
     • Then are you sure that you phosphorylated the primers? The Knight protocol doesn't call for this step.

• I'm sure we did.

1. Do we have the regulated wintergreen GC data in a form that we could generate plots similar to the banana timecourse?

• Yes, we could. It would take some digging through old files and sorting out through each protocol we used on each day. I don't know if it's necessary to show though.

1. What did you use to shake the cultures? i.e. what incubator or shaker?

220 RPM- the shaker in 37 C room

110 RPM- the shaker by Samantha's/2007 iGEM team's bench

General outstanding issues

• Funding sources for each person
• Protocol for transformation
• Describing figure 6
• Update <bbpart>BBa_Q04401</bbpart>