Talk:Kubke Lab:Research/CND/Records/MH008: Difference between revisions
No edit summary |
(Trying to add explanations of annotations.) |
||
| Line 8: | Line 8: | ||
Both links you provide link to the same webpage. What I mean by criterion is on what basis are you deciding what each structure you identify. When you say that you compare the morphology, you need to be more specific. For example, why are you deciding that what you have labelled as the optic cup is the optic cup? What other structure could it be, how would you differentiate between those, is the morphology you see consistent with what you would expect for the age of the embryo you are looking at, etc. | Both links you provide link to the same webpage. What I mean by criterion is on what basis are you deciding what each structure you identify. When you say that you compare the morphology, you need to be more specific. For example, why are you deciding that what you have labelled as the optic cup is the optic cup? What other structure could it be, how would you differentiate between those, is the morphology you see consistent with what you would expect for the age of the embryo you are looking at, etc. | ||
Explanations for annotations: | |||
The eye originates in the wall of the diencephalon (Carlson, 1999). I think I have labeled the optic cup incorrectly. Accoridng to Carlson, 1999 the optic vesicles become the optic cup following induction of the lens. In this section I think I can only see the pigment and sensory layers of the optic vesicle. There is a SEM photogprah on pg 264 and a diagram on pg 263 in Carlson, 1999 which shows the distinctive continuous double layered morphology of the developing optic vesicle. | |||
The dienncephalon is recognizable by the prominent lateral optic vesicles extending from its walls (Carlson, 1999). So a cross section through the diencephalon should show symmetrical optic vesicles on either side. | |||
Due to the cephalic and cervical flexures the developing brain doubles back on itself and in certain cross sections you wll be able to see the brain appear twice in the section. | |||
Revision as of 16:24, 10 February 2011
.jpg)
Please discuss the accuracy of my annotations, cheers. --Reuben Cutfield 21:12, 8 February 2011 (EST)
Please provide references and the criteria you used to identify the structures --MF Kubke 22:32, 8 February 2011 (EST)
This is a photograph of a section from MH008. I was looking through several books in the library and then a few websites to help. Fact is I found nothing that specifically had what I was looking for. One website (click here) had cross sections but in a different plane and I had to interpret the sections using the plane our embryo was cut with. I also looked at photos of embryos I had taken such as the one in MH007 to imagine what structures would be included in each section and what the exact plane of our cutting was. Another website I found useful was this one. Not sure what you are asking in terms of criteria. I was just comparing the morphology of different structures to those in diagrams whilst thinking about the angle we cut at. I think it will be easier to complete the labeling of all my section once I have confirmation that I am seeing what I think I am seeing and have a few structures labeled in concrete. Thanks.--Reuben Cutfield 01:59, 9 February 2011 (EST)
Both links you provide link to the same webpage. What I mean by criterion is on what basis are you deciding what each structure you identify. When you say that you compare the morphology, you need to be more specific. For example, why are you deciding that what you have labelled as the optic cup is the optic cup? What other structure could it be, how would you differentiate between those, is the morphology you see consistent with what you would expect for the age of the embryo you are looking at, etc.
Explanations for annotations:
The eye originates in the wall of the diencephalon (Carlson, 1999). I think I have labeled the optic cup incorrectly. Accoridng to Carlson, 1999 the optic vesicles become the optic cup following induction of the lens. In this section I think I can only see the pigment and sensory layers of the optic vesicle. There is a SEM photogprah on pg 264 and a diagram on pg 263 in Carlson, 1999 which shows the distinctive continuous double layered morphology of the developing optic vesicle.
The dienncephalon is recognizable by the prominent lateral optic vesicles extending from its walls (Carlson, 1999). So a cross section through the diencephalon should show symmetrical optic vesicles on either side.
Due to the cephalic and cervical flexures the developing brain doubles back on itself and in certain cross sections you wll be able to see the brain appear twice in the section.
