Talk:Materials

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(live cell stains)
(live cell stains)
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There a number of dyes from [http://probes.invitrogen.com/handbook/sections/1503.html Molecular Probes] that are supposedly pretty good at staining live cells a particular color.   
There a number of dyes from [http://probes.invitrogen.com/handbook/sections/1503.html Molecular Probes] that are supposedly pretty good at staining live cells a particular color.   
For example, their BacLight Bacterial Membrane Potential kit uses a dye DiOC2 that is green in all cells, but turns red upon concentration at the cell membrane if there is an active proton gradient.  Also, there are dyes that measure redox potential of live cells through reduction of [http://probes.invitrogen.com/servlets/product?item=34951 C12-resazurin]
For example, their BacLight Bacterial Membrane Potential kit uses a dye DiOC2 that is green in all cells, but turns red upon concentration at the cell membrane if there is an active proton gradient.  Also, there are dyes that measure redox potential of live cells through reduction of [http://probes.invitrogen.com/servlets/product?item=34951 C12-resazurin]
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== live cell stains ==
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There a number of dyes from [http://probes.invitrogen.com/handbook/sections/1503.html Molecular Probes] that are supposedly pretty good at staining live cells a particular color. 
 +
For example, their BacLight Bacterial Membrane Potential kit uses a dye DiOC2 that is green in all cells, but turns red upon concentration at the cell membrane if there is an active proton gradient.  Also, there are dyes that measure redox potential of live cells through reduction of [http://probes.invitrogen.com/servlets/product?item=34951 C12-resazurin] --[[User:Skosuri|Skosuri]] 14:27, 29 Apr 2005 (EDT)

Revision as of 14:27, 29 April 2005

I added some notes collected previously on various dyes and stains that we could potentially use in flow cytometry.

live cell stains

There a number of dyes from Molecular Probes that are supposedly pretty good at staining live cells a particular color. For example, their BacLight Bacterial Membrane Potential kit uses a dye DiOC2 that is green in all cells, but turns red upon concentration at the cell membrane if there is an active proton gradient. Also, there are dyes that measure redox potential of live cells through reduction of C12-resazurin

live cell stains

There a number of dyes from Molecular Probes that are supposedly pretty good at staining live cells a particular color. For example, their BacLight Bacterial Membrane Potential kit uses a dye DiOC2 that is green in all cells, but turns red upon concentration at the cell membrane if there is an active proton gradient. Also, there are dyes that measure redox potential of live cells through reduction of C12-resazurin --Skosuri 14:27, 29 Apr 2005 (EDT)

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