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(Are the masses for the phosphates for anhydrous versions or hydrated?)
Current revision (09:15, 22 April 2009) (view source)
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==Anhydrous?==
==Anhydrous?==
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Are the masses for the phosphates for anhydrous versions or hydrated?  *'''[[User:Nessie Van Loan|boo_pixie@yahoo.com]] 19:52, 21 April 2009 (EDT)''':
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Are the masses for the phosphates for anhydrous versions or hydrated?  *'''[[User:Nessie Van Loan|Nessie]] 19:52, 21 April 2009 (EDT)''':
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==Reply==
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*'''[[User:Torsten Waldminghaus|Torsten Waldminghaus]] 09:09, 22 April 2009 (EDT)''': According to Sambrook they are anhydrous. However, I calculated the concentration of phosphates than and it is 12 mM instead of 10 mM. I changed that in the protocol and added the gram you need if you have 2H<sub>2</sub>O hanging on your Na<sub>2</sub>HPO<sub>4</sub>. If anybody has phosphates with other H<sub>2</sub>O please insert the ammount to be used. For helping with calculation here are the molecular masses:
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*Na<sub>2</sub>HPO<sub>4</sub>: 141.96 g/mol
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*KH<sub>2</sub>PO<sub>4</sub>: 136.09 g/mol
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*H<sub>2</sub>O: 18 g/mol

Current revision

Anhydrous?

Are the masses for the phosphates for anhydrous versions or hydrated? *Nessie 19:52, 21 April 2009 (EDT):

Reply

  • Torsten Waldminghaus 09:09, 22 April 2009 (EDT): According to Sambrook they are anhydrous. However, I calculated the concentration of phosphates than and it is 12 mM instead of 10 mM. I changed that in the protocol and added the gram you need if you have 2H2O hanging on your Na2HPO4. If anybody has phosphates with other H2O please insert the ammount to be used. For helping with calculation here are the molecular masses:
  • Na2HPO4: 141.96 g/mol
  • KH2PO4: 136.09 g/mol
  • H2O: 18 g/mol
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