Talk:PBS

Anhydrous?

Are the masses for the phosphates for anhydrous versions or hydrated? *Nessie 19:52, 21 April 2009 (EDT):

Reply

• Torsten Waldminghaus 09:09, 22 April 2009 (EDT): According to Sambrook they are anhydrous. However, I calculated the concentration of phosphates than and it is 12 mM instead of 10 mM. I changed that in the protocol and added the gram you need if you have 2H₂O hanging on your Na₂HPO₄. If anybody has phosphates with other H₂O please insert the ammount to be used. For helping with calculation here are the molecular masses:
• Na₂HPO₄: 141.96 g/mol
• KH₂PO₄: 136.09 g/mol
• H₂O: 18 g/mol