Talk:Protocols

This is a proposed restyling of the protocols page. I thought I would put it here to see what people thought before making it go live on the real page. Comments welcome. I just thought the page could be made more compact and easy to view. Feel free to retweak if you have a better idea. A similar thing has been suggested for the materials page. My only concern is whether it makes it more difficult/intimidating for those new to wikis to edit? Which is definitely not what I want. -- RS

I made a protocols template. It has all the formatting from the layout below. Power users can edit the template to add new categories, formats etc. This page has the new version of the protocols using that template. This makes it a tiny bit easier for people to edit the protocols list without dealing with all the formatting. This is good but it means that users need to go edit the template page if they want to add new categories so there is trade off between making some editing simple and higher level editing more difficult.

It looks like the ease of editing afforded by using the template as described above probably doesn't merit the extra complexity of having templates as well as pages. It might however be beneficial if the protocols, materials, help pages etc, were all formatted in a common way. Then you could just change the template and style changes would be applied to all pages.--BC 12:14, 11 January 2006 (EST)

• agreed on both points - template probably not worth it for the page, though eventually moving to a common template would be pretty nice. Also, this is WAY better, I think we should go live with this ASAP. Though might be nice to have a graphic saying 'protocols' beneath the OWW logo...-- JK

• yeah that was Austin's objection to the page as well. That it looked TOO similar to the frontpage. I just didn't know how the openwetware graphic was made and if we could easily make a "Protocols" graphic to go under it and similarly for the Materials page. As for the templating, I kind of like it. It does make things easier to look at. -- RS

• I was going to suggest removing the OWW logo altogether. Maybe replacing it with a protocols graphic will work, but most people will have navigated their way here, so they'll know what they're looking at. I think the template may be bit too complicated for now and may inhibit the addition of new protocols to the shared page. I also don't think the categories we have now are anywhere near "all-encompassing" especially given that some real biology labs are joining up now. I do agree that a template to standardizing formatting will be a good thing in the future, though.--Kathleen 15:22, 11 January 2006 (EST)
  • i don't like the implications of your "real biology labs" comment, kathleen ;) --JK
  • I was talking about our lab, too. Some of these people work with what most people in the world would consider as critters, not the single-celled guys we play around with. Didn't mean to offend. :)--Kathleen 16:56, 11 January 2006 (EST)

In vivo Escherichia coli beta-Galactosidase Assay (A better Miller) Bacterial cell culture Chemically competent cells Electrocompetent cells Electroporation Miniprep Colony PCR Pulse-chase protein production Pour LB plates S_N Chemostat Protocol Yeast High Efficiency Transformation Yeast Colony PCR Yeast DNA Prep Cell Cycle Arrest Fixing cells B-galactosidase assay Time-Lapse Microscopy Simple Yeast Lysate (for Western etc.) Assaying mating Pour YPD plates Dropout plates for yeast Layered plates T7 Simple lysate protocol from plaque or lysate Cesium Chloride Purification of T7 Mesoplasma florum Culture Media

In vitro Nucleic acids Quantification of nucleic acids Phenol/chloroform extraction Nucleic acid precipitation Denaturing acrylamide gel purification of nucleic acids DNA Agarose gel electrophoresis Annealing Primers Designing primers DNA Ligation Library Generation Miniprep PCR Phosphatase treatment of linearized vector PNK Treatment of DNA Ends Purification of DNA Reconstituting primers Restriction digest 'Round-the-horn site-directed mutagenesis Sequencing DNA Site-directed mutagenesis Templiphi RNA RNA Extraction Northern Blot, 32P End-Labeled Probes Probe Prep, 32P End-Labeled Probes RNase Protection Assay In vitro transcription with T7 RNA polymerase RNA Half-life Protein Acrylamide gels and SDS-PAGE Coomassie Stain Coomassie Destain Silver staining Western Blot HPLC

Other Microfluidics Design a Microfluidic Chip Order a Microfluidic Chip from the Foundry Operate Microfluidic Chemostat Microscopy Agarose Pads for Microscopy Quantitative Microscopy Time-Lapse Microscopy Flow Cytometry Flow Calibration Plate reader Counteracting evaporation from wells in a Victor3 plate reader