Talk:RNA extraction using trizol/tri

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(reply to Leo)
Current revision (07:57, 22 February 2009) (view source)
(About Trizol: reply)
 
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==About Trizol==
==About Trizol==
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I want to make some homemade Trizol. However I find something very wired in the protocols in Openwetware([[http://openwetware.org/wiki/RNA_extraction_using_trizol/tri]]). In protocols Guanidinium Thioyanate(W.M. 118.2) final concentration is 0.8M, but 118.2g is used in 1L Trizol. Ammonium thiocyanate (W.M. 76.1) is 76.1g in Trizol, however, they said the final concentration is 0.4M. I can not understant that. Thank you for your answer!
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I want to make some homemade Trizol. However I find something very wired in the protocols in Openwetware([[http://openwetware.org/wiki/RNA_extraction_using_trizol/tri]]). In protocols Guanidinium Thioyanate(W.M. 118.2) final concentration is 0.8M, but 118.2g is used in 1L Trizol. Ammonium thiocyanate (W.M. 76.1) is 76.1g in Trizol, however, they said the final concentration is 0.4M. I can not understant that. Thank you for your answer! [[User:Leo Tang|Leo Tang]]
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[[User:Leo Tang|Leo Tang]]
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: Well spotted Leo. I copied this section from a colleague and didn't spot the mistake since I buy my TRI/Trizol. But this is what OpenWetWare is all about. You spot a mistake, correct it directly, or contact your colleagues on OWW. I put a protocol for your making your own reagents here: [[RNA extraction using self-made guanidinium-acid-phenol reagents]]. It's based on the Chomczynski publications. All the best, [[User:Jakob Suckale|Jakob]] 07:56, 22 February 2009 (EST)
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: Well spotted Leo. I copied this section from a colleague and didn't spot the mistake since I buy my TRI/Trizol. But this is what OpenWetWare is all about. You spot a mistake, correct it directly, or contact your colleagues on OWW. I put a protocol for your making your own reagents here: [[RNA extraction using self-made guanidinium-acid-phenol reagents]]. It's based on the Chomczynski publications. All the best, [[User:Jakob Suckale|Jakob Suckale]] 07:56, 22 February 2009 (EST)
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Current revision

About Trizol

I want to make some homemade Trizol. However I find something very wired in the protocols in Openwetware([[1]]). In protocols Guanidinium Thioyanate(W.M. 118.2) final concentration is 0.8M, but 118.2g is used in 1L Trizol. Ammonium thiocyanate (W.M. 76.1) is 76.1g in Trizol, however, they said the final concentration is 0.4M. I can not understant that. Thank you for your answer! Leo Tang

Well spotted Leo. I copied this section from a colleague and didn't spot the mistake since I buy my TRI/Trizol. But this is what OpenWetWare is all about. You spot a mistake, correct it directly, or contact your colleagues on OWW. I put a protocol for your making your own reagents here: RNA extraction using self-made guanidinium-acid-phenol reagents. It's based on the Chomczynski publications. All the best, Jakob 07:56, 22 February 2009 (EST)
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