Talk:Restriction Digest (Biobricks): Difference between revisions
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'''Reaction Volumes''' - I used to have very inconsistent results when digesting with EcoR1, using the 20μl reaction volume. Using the Knight Lab's 50μl mix has recently given good results. I haven't tested this enough to say it is statistically significant. --[[User:Bcanton|BC]] 13:39, 2 Jun 2005 (EDT) | '''Reaction Volumes''' - I used to have very inconsistent results when digesting with EcoR1, using the 20μl reaction volume. Using the Knight Lab's 50μl mix has recently given good results. I haven't tested this enough to say it is a statistically significant result. --[[User:Bcanton|BC]] 13:39, 2 Jun 2005 (EDT) |
Latest revision as of 10:41, 2 June 2005
Reaction Volumes - I used to have very inconsistent results when digesting with EcoR1, using the 20μl reaction volume. Using the Knight Lab's 50μl mix has recently given good results. I haven't tested this enough to say it is a statistically significant result. --BC 13:39, 2 Jun 2005 (EDT)