Talk:Shreffler:CFSE Labeling
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Current revision (23:45, 19 May 2009) (view source) m (Talk:CFSE Labeling moved to Talk:Shreffler:CFSE Labeling: lab protocol) |
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| - | + | <div style="padding: 10px; width: 720px; border: 5px solid #000000;"> | |
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| + | Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input! | ||
| + | #List troubleshooting tips here. | ||
| + | #You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites. | ||
| + | #Anecdotal observations that might be of use to others can also be posted here. | ||
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| + | Please sign your name to your note by adding <font face="courier"><nowiki>'''*~~~~''':</nowiki></font> to the beginning of your tip. | ||
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| + | </div> | ||
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| + | == CFSE toxicity == | ||
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| + | I have noticed that higher concentrations of CFSE (10 μM) may suppress some lymphocyte function and the user should experiment with optimal labeling concentration for their experiment | ||
| + | *'''[[User:Wayne G. Shreffler|Wayne G. Shreffler]] 22:28, 19 May 2009 (EDT)''': | ||
Current revision
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
- List troubleshooting tips here.
- You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
- Anecdotal observations that might be of use to others can also be posted here.
Please sign your name to your note by adding '''*~~~~''': to the beginning of your tip.
CFSE toxicity
I have noticed that higher concentrations of CFSE (10 μM) may suppress some lymphocyte function and the user should experiment with optimal labeling concentration for their experiment
- Wayne G. Shreffler 22:28, 19 May 2009 (EDT):
