Talk:Shreffler:CFSE Labeling: Difference between revisions
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Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input! | Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input! | ||
#List troubleshooting tips here. | #List troubleshooting tips here. | ||
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Please sign your name to your note by adding <font face="courier"><nowiki>'''*~~~~''':</nowiki></font> to the beginning of your tip. | Please sign your name to your note by adding <font face="courier"><nowiki>'''*~~~~''':</nowiki></font> to the beginning of your tip. | ||
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== CFSE toxicity == | |||
I have noticed that higher concentrations of CFSE (10 μM) may suppress some lymphocyte function and the user should experiment with optimal labeling concentration for their experiment | |||
*'''[[User:Wayne G. Shreffler|Wayne G. Shreffler]] 22:28, 19 May 2009 (EDT)''': | |||
Latest revision as of 20:45, 19 May 2009
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
- List troubleshooting tips here.
- You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
- Anecdotal observations that might be of use to others can also be posted here.
Please sign your name to your note by adding '''*~~~~''': to the beginning of your tip.
CFSE toxicity
I have noticed that higher concentrations of CFSE (10 μM) may suppress some lymphocyte function and the user should experiment with optimal labeling concentration for their experiment
- Wayne G. Shreffler 22:28, 19 May 2009 (EDT):
