Talk:Standard E. coli Strain for BioBricks: Difference between revisions
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A paper that I ran across - "Growth-rate recovery of Escherichia coli cultures carrying a multicopy plasmid, by engineering of the pentose-phosphate pathway.", by Flores et al. | A paper that I ran across - "Growth-rate recovery of Escherichia coli cultures carrying a multicopy plasmid, by engineering of the pentose-phosphate pathway.", by Flores et al. | ||
Short version - they overexpressed G6PDH and largely restored the growth rate of a strain carrying a high copy plasmid. Doesn't solve the problem of protein load, but it might be useful in a BB strain, particularly for constructing (non-functional or non-induced) intermediates. -- Josh | Short version - they overexpressed G6PDH and largely restored the growth rate of a strain carrying a high copy plasmid. Doesn't solve the problem of protein load, but it might be useful in a BB strain, particularly for constructing (non-functional or non-induced) intermediates. -- Josh | ||
I have a folder with all the work that Bram and Caitlin did on building the KanR/PheS expression cassette, which I can pass on to anyone who wants to use it in the short term --[[User:Bcanton|BC]] 16:36, 8 Jun 2005 (EDT) | |||
:Can this information be loaded into the wiki easily? --[[User:Rshetty|Reshma]] 20:00, 8 Jun 2005 (EDT) | |||
From F. R. Blattner et al. The Complete Genome Sequence of Escherichia coli K-12 ''Science'', 277(5331):1453-1462, 1997 | |||
: We chose strain MG1655 as the representative to sequence because it has been maintained as a laboratory strain with minimal genetic manipulation, having only been cured of the temperate bacteriophage lambda and F plasmid by ultraviolet light and acridine orange, respectively. --[[User:Jgritton|Jgritton]] 11:30, 11 Jul 2005 (EDT) | |||
Latest revision as of 08:30, 11 July 2005
A paper that I ran across - "Growth-rate recovery of Escherichia coli cultures carrying a multicopy plasmid, by engineering of the pentose-phosphate pathway.", by Flores et al. Short version - they overexpressed G6PDH and largely restored the growth rate of a strain carrying a high copy plasmid. Doesn't solve the problem of protein load, but it might be useful in a BB strain, particularly for constructing (non-functional or non-induced) intermediates. -- Josh
I have a folder with all the work that Bram and Caitlin did on building the KanR/PheS expression cassette, which I can pass on to anyone who wants to use it in the short term --BC 16:36, 8 Jun 2005 (EDT)
- Can this information be loaded into the wiki easily? --Reshma 20:00, 8 Jun 2005 (EDT)
From F. R. Blattner et al. The Complete Genome Sequence of Escherichia coli K-12 Science, 277(5331):1453-1462, 1997
- We chose strain MG1655 as the representative to sequence because it has been maintained as a laboratory strain with minimal genetic manipulation, having only been cured of the temperate bacteriophage lambda and F plasmid by ultraviolet light and acridine orange, respectively. --Jgritton 11:30, 11 Jul 2005 (EDT)
