Talk:SynBERC:COG
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QiaCube
The Knight lab did a 1 day evaluation of the QiaCube.
Here are some observations and results...
- The yield from a 5mL LB culture prep of pUC19 was 80-85 ng/μL using the default QiaPrep Miniprep protocol with an single elution volume of 50 μL.
- The yield from an 8mL LB culture prep of pSB3T5-I52001 (p15A origin) was 30 ng/μL using the default QiaPrep Miniprep protocol with an single elution volume of 50 μL.
- It costs about $0.13 per sample in additional plastic (beyond the kit itself).
- A miniprep of 8 samples takes about 50 minutes, 12 samples takes about 58 minutes.
- 12 sample max
Advantages
- Standardization
- Save time by not doing the prep yourself
Disadvantages
- Currently the minimum elution volume for a double elution is 50 μL for each elution (this may be fixable). The minimum elution volume for a single elution is 30 μL.
- Each new protocol variant costs $400 and has a 10 day turnaround time (potentially addressable). Totally new protocols may cost more and take more time.
- You can't heat the elution buffer (which can improve yield).
- The robot is incapable of reloading flow through onto a column a second time (useful for low copy minipreps).
- You can't save copies of existing protocols with different settings.
Outstanding issues
- Could we go directly from a miniprep to a digestion step using this robot? This would definitely require a custom protocol but it is not clear that the robot can do this.