Talk:Synthetic Biology:Vectors

Vector design review meeting

We will be holding a meeting to review the proposed design for some new BioBricks standard vectors.

Scheduling

Please list your name beneath any of the suggested times below that you CAN attend.

• Friday March 10, 12:00pm-1:30pm
  1. RS
  2. BC
  3. Austin Che
  4. Tom Knight I have class until noon; may be a bit late, best time for me
  5. cmc
• Monday March 13, 3:30pm-5pm
  1. RS
  2. BC
  3. Austin Che
  4. Tom Knight Could do this, would prefer not
  5. RR
  6. cmc
• Wednesday March 15, 3:30pm-5pm
  1. RS
  2. BC
  3. Austin Che
  4. Tom Knight
  5. RR
  6. cmc
  7. <insert name here>
• None of the above
  1. DE (should be able to meet on Wed time but need to reschedule another meeting)
  2. Jkm
  3. <insert name here>

Issues to be discussed

• Are there any other features that we want in these vectors?
• Plasmid barcode
  • How can we get this to a final stage?
  • Does this require a more significant investment of resources?
• How can we "verify" the design?
• How should the vectors be fabricated?
  • Direct synthesis of complete vectors
    • expensive
    • synthesis problems are dealt with by company not us
  • Direct synthesis of necessary parts with manual assembly
    • less expensive
    • very time-consuming and labor intensive
    • risks not getting done
  • Direct synthesis of complete vectors and individual parts
    • very expensive (how much more?)
    • enables construction of new variants later
• Others?

Relevant pages

• BioBricks parts
• Single copy plasmid
• Synthetic Biology:Vectors/Barcode