Talk:Synthetic Biology:Vectors: Difference between revisions
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==Vector design review meeting== | ==Vector design review meeting== | ||
We | We held a meeting to review the proposed design for some new BioBricks standard vectors. | ||
=== | ===Meeting notes=== | ||
*Are there any other features that we want in these vectors? | *Are there any other features that we want in these vectors? | ||
**All BioBricks compatible restriction sites should be removed from the vectors once the resistance marker and replication origin have been inserted. | |||
**Only include a single antibiotic resistance marker (rather than two). | |||
**Include a unique restriction site in the backbone for situations where the insert is equal in size to the backbone (such that they can be distinguished). | |||
**Since all components will be synthesized, remove unnecessary restriction sites. Also do codon optimization? See [[Codon pairs]]. | |||
*Should the flanking terminators be inside or outside the verification primer binding sites? | |||
**Inside. | |||
*[[Synthetic Biology:Vectors/Barcode | Plasmid barcode]] | *[[Synthetic Biology:Vectors/Barcode | Plasmid barcode]] | ||
** | **Use a 3-4 bp tag that can be read during sequencing. Also place this tag immediately 3' to VF2 and VR so that vector specific primers can be used in single colony PCR? | ||
*How can we "verify" the design? | *How can we "verify" the design? | ||
* | **Caitlin, Barry and TK have volunteered to inspect the design. | ||
*How should the vectors be fabricated? | |||
**Direct synthesis of useful vector components, scaffold and intact vectors (budget of $20,000). Prepare a flow chart of synthesis and cloning steps so that we can obtain useful intermediates and test whether they work or not. | |||
===Relevant pages=== | ===Relevant pages=== | ||
| Line 36: | Line 22: | ||
*[[Synthetic Biology:Vectors/Single copy plasmid | Single copy plasmid]] | *[[Synthetic Biology:Vectors/Single copy plasmid | Single copy plasmid]] | ||
*[[Synthetic Biology:Vectors/Barcode]] | *[[Synthetic Biology:Vectors/Barcode]] | ||
*[[Codon pairs]] | |||
Latest revision as of 09:56, 31 May 2006
Vector design review meeting
We held a meeting to review the proposed design for some new BioBricks standard vectors.
Meeting notes
- Are there any other features that we want in these vectors?
- All BioBricks compatible restriction sites should be removed from the vectors once the resistance marker and replication origin have been inserted.
- Only include a single antibiotic resistance marker (rather than two).
- Include a unique restriction site in the backbone for situations where the insert is equal in size to the backbone (such that they can be distinguished).
- Since all components will be synthesized, remove unnecessary restriction sites. Also do codon optimization? See Codon pairs.
- Should the flanking terminators be inside or outside the verification primer binding sites?
- Inside.
- Plasmid barcode
- Use a 3-4 bp tag that can be read during sequencing. Also place this tag immediately 3' to VF2 and VR so that vector specific primers can be used in single colony PCR?
- How can we "verify" the design?
- Caitlin, Barry and TK have volunteered to inspect the design.
- How should the vectors be fabricated?
- Direct synthesis of useful vector components, scaffold and intact vectors (budget of $20,000). Prepare a flow chart of synthesis and cloning steps so that we can obtain useful intermediates and test whether they work or not.
