Talk:The BioBricks Foundation:Standards/Technical/Measurement/Promoter characterization experiment (FACS): Difference between revisions
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*We only spun-down and resuspended 1ml of each sample in PBS. | *We only spun-down and resuspended 1ml of each sample in PBS. | ||
*We also only did one wash with PBS, so I'm updating the protocol for future labs doing this -- since Caroline made the same choice :) | *We also only did one wash with PBS, so I'm updating the protocol for future labs doing this -- since Caroline made the same choice :) | ||
*Our [https://catalog.invitrogen.com/index.cfm?fuseaction=viewCatalog.viewProductDetails&sku=&productDescription=87&CID=AFLBC,AFL-BIOCOMPARE&ref=http%3A%2F%2Fwww%2Ebiocompare%2Ecom%2Fitemdetails%2Easp%3Fitemid%3D5705 PBS is from invitrogen] and looks like it has a different formulation then what caroline listed in materials. | |||
Revision as of 23:13, 25 March 2008
LBL: Effects of Varying the Growth conditions on the Promoter characterization experiment
- Because of my poor planning, we made the following modifications to the procedure, resulting in relatively minor differences in the ratio of GFP intensities:
- Culturing the strains
- grew in LB + Kan overnight
- grew at 37C shaking at 225 rpm
- back diluted 1:1000 (instead of 1:100 doh!!) supplemented M9 media, and grew for 4 hours so OD600<0.1
- Live Cell FACS Procedure
- washed only once with PBS
- Culturing the strains
- CAjoF 17:45, 5 March 2008 (EST)
MIT: Experimental details
- We only spun-down and resuspended 1ml of each sample in PBS.
- We also only did one wash with PBS, so I'm updating the protocol for future labs doing this -- since Caroline made the same choice :)
- Our PBS is from invitrogen and looks like it has a different formulation then what caroline listed in materials.
