Talk:Transforming chemically competent cells: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
(→Troubleshooting: response) |
||
| (3 intermediate revisions by one other user not shown) | |||
| Line 5: | Line 5: | ||
--[[User:Mathias Klode|Mathias Klode]] 08:24, 16 April 2009 (EDT) | --[[User:Mathias Klode|Mathias Klode]] 08:24, 16 April 2009 (EDT) | ||
== Troubleshooting == | |||
In my hands, shaking can reduce the transformation efficiency, specially for Ampicillin resistance. | |||
*'''[[User:Modesto Redrejo Rodriguez|zurdo]] 07:06, 28 April 2009 (EDT)''': | |||
Shaking at which stage exactly? And I don't think there is an interdependency between transformation efficiency, antibiotic and ''shaking''... (damn I forgot how to change indentation) --[[User:Mathias Klode|Mathias Klode]] 07:23, 28 April 2009 (EDT) | |||
Latest revision as of 04:23, 28 April 2009
Amount of plasmid DNA used
I have a few suggestions:
- the amount of plasmid used should better be posted in µg. Are there any recommendations for the exact amount?
- There are a lot of abbreviations (TSS) used. maybe this should be clarified as well.
--Mathias Klode 08:24, 16 April 2009 (EDT)
Troubleshooting
In my hands, shaking can reduce the transformation efficiency, specially for Ampicillin resistance.
- zurdo 07:06, 28 April 2009 (EDT):
Shaking at which stage exactly? And I don't think there is an interdependency between transformation efficiency, antibiotic and shaking... (damn I forgot how to change indentation) --Mathias Klode 07:23, 28 April 2009 (EDT)
