Talk:Transforming chemically competent cells: Difference between revisions
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--[[User:Mathias Klode|Mathias Klode]] 08:24, 16 April 2009 (EDT) | --[[User:Mathias Klode|Mathias Klode]] 08:24, 16 April 2009 (EDT) | ||
== Troubleshooting == | |||
In my hands, shaking can reduce the transformation efficiency, specially for Ampicillin resistance. |
Revision as of 04:05, 28 April 2009
Amount of plasmid DNA used
I have a few suggestions:
- the amount of plasmid used should better be posted in µg. Are there any recommendations for the exact amount?
- There are a lot of abbreviations (TSS) used. maybe this should be clarified as well.
--Mathias Klode 08:24, 16 April 2009 (EDT)
Troubleshooting
In my hands, shaking can reduce the transformation efficiency, specially for Ampicillin resistance.