Team:Chiba/Sender experiments/Senders(JW1908) T9002(JW1908)
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Results and Discussion
Reaction temparature:37°C,09/12
Sender culture：1000μL,Receiver culture:1000μL
12/09.E.coli strain,senders:JW1908,BBa_T9002:JW1908,Reaction temparature:37°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.
Sender culture：100μL,Receiver culture：1000μL
E.coli strain,senders:JW1908,BBa_T9002:JW1908,37°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.
- Fig. 1,Fig. 2
- Results
- Green fluorescence intensity didn't increase in the culture containing BBa_K0840010(plac+CinI+LVA).We thought BBa_K084010 didn't work properly or LuxR gene didn't interact with signal molecules synthesized by BBa_K084010.
- Others responded at the same time(4 hours after induction).Final fluorescence intensity differd.
Reaction temparature:37°C
Sender culture：500μLm,Receiver culture：500μL
E.coli strain,senders:JW1908,BBa_T9002:JW1908,37°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.
E.coli strain,senders:JW1908,BBa_T9002:JW1908,37°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.
- Fig. 3
- Results
- Response time and final fluorescence intensity showed no significant difference.
- Discussion
- We thought AHL concentration was quickly reached the threshold concentration.The fluorescence intensity increased as gfp maturured.
- Fig. 4
- Results
- No significant difference in fluorescence intensity between the culture containing BBa_K084007(plac+RhlI) gene transformed cells and the culture containing BBa_K084008(plac+RhlI(LVA)) gene transformed cells.
- Discussion
- We thought that the rate of AHL synthesis by each autoinducer synthase was much faster than the rate of its degradation by protease.
Reaction temparature:30°C
Sender culture：500μL,Receiver culture：500μL
E.coli strain,Senders:JW1908,BBa_T9002:JW1908,30°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.
E.coli strain,Senders:JW1908,BBa_T9002:JW1908,30°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.
- Fig. 6
- Results
- No significant difference in fluorescence intensity between the cultures containing BBa_K084007(plac+RhlI) gene transformed cells and the culture containing BBa_K084008(plac+RhlI(LVA)) gene transformed cells.
- Discussion
- We thought that the rate of AHL synthesis by each autoinducer synthase was much faster than the rate of its degradation by protease.
Sender culture：100μL,Receiver culture：1000μL
E.coli strain,senders:JW1908,BBa_T9002:JW1908,30°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.
E.coli strain,senders:JW1908,BBa_T9002:JW1908,30°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.
- Fig.7
- Results
- No significant difference in fluorescence intensity between the culture containing BBa_K084007(plac+RhlI) gene transformed cells and the culture containing BBa_K084008(plac+RhlI(LVA)) gene transformed cells.
- Discussion
- We thought that the rate of AHL synthesis by each autoinducer synthase was much faster than the rate of its degradation by protease.
Sender culture：10μL,Receiver culture：1000μL
E.coli strain,senders:JW1908,BBa_T9002:JW1908,30°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.
- Fig.9
- Results
- The final fluorescence intensity of the culture containing BBa_K084008 transformed cells which express LVA-tagged RhlI protein was lower than the culture containing cells which express untagged RhlI protein.It shows that LVA-tagged RhlI protein was degraded by LVA-specific protase.However,fluorescence intensity of both cultures increased at the same time.
- Discussion
- We thought it was because the AHL concentration was quickly reached the threshold concentration and cells began to express gfp.After 2 hours,fluorescence intensity started to increase.
