Team kansai project2: Difference between revisions
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== <font face="Helvetica" color="#298cda">Previous experiments</font> == | == <font face="Helvetica" color="#298cda">Previous experiments</font> == | ||
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Our previous strategy of the robot contest was using a ditch. | Our previous strategy of the robot contest was making a walking system using a ditch. | ||
Self-walking molecular spider (Fig. 1) that we called “Molecular NINJA” because walking in a ditch is like NINJA walks on water, was selected as the molecular walking system. | |||
It is reported that it is possible to tightly trap a guest using well. So the walker can walks in a ditch without suffering damage from physical tapping by AFM probe. | It is reported that it is possible to tightly trap a guest using well. So the walker can walks in a ditch without suffering damage from physical tapping by AFM probe. | ||
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Revision as of 03:28, 26 October 2012
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| Top | Team | Project | Results | Protocols | photos |
Abstract
We presented“Molecular NINJA ”system at Biomod 2011. It is a system of molecular walker which walks in a ditch made of two DNA origamis.
We designed DNA origamis which has a ditch that the molecular NINJA
can hide in and guides its route. However, we were not able to achieve this plan. We will take revenge of last year's project. Our project title is"Molecular NINJA Returns".
Key point of the project is a metamorphosis like ninja.
We prepare without the addition of one row of vertical staple DNAs that
make up the DNA Origami.There are bare base pairs in the space of lacking staples. Stacking interaction occurs between them and shrink the original structure. Using this phenomenon, we attempt to construct a DNA origami which has a capability of transformation like using "ninjutsu".
Previous experiments
Our previous strategy of the robot contest was making a walking system using a ditch.
Self-walking molecular spider (Fig. 1) that we called “Molecular NINJA” because walking in a ditch is like NINJA walks on water, was selected as the molecular walking system.
It is reported that it is possible to tightly trap a guest using well. So the walker can walks in a ditch without suffering damage from physical tapping by AFM probe.
The detailed method was as follows (Fig. 2).
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Molecular NINJA Returns -Shrunk origami-
In this project, DNA origami can be transformed like NINJA's skill, "ninjutsu". We design the transforming DNA Origami. The transformation is based on π- π stacking interaction between intramolecular blant-ends. By making DNA origami without staple strands on few vertical lines, shrunk DNA origami is formed.
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Cyclops ⇒ Smiley DNA origami
This image is popular smiley DNA origami. Not using staple strands shown in center, center fell out smiley DNA origami formed the one-eyed monster called “Cyclops” by intramolecular-stacking. We named cyclops DNA origami. Adding staple strands, so cyclops DNA origami is expected to transform the smiley DNA origami.
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Bear Trap DNA origami -The appearing stage-
If above method is used, it will be possible to make DNA origami having a capability of shape selective capturing. We designed shape selective capturing DNA origami which is consist of two U shaped regions connected by single stranded DNA. This architecture will form Origami with the square hole in the center by π- π stacking interaction. However, this DNA origami might be able to select catch another complementary shaped DNA origami to maximize π- π stacking interaction.
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