Temperature mixing formula
This page is to help calculating the amount of one liquid you need to add to another to get a special temperature. This is often usefull if you perform a temperature shift for example with cultures of organisms that carry a temperature sensitive mutant.
Here is a form of Richmann’s calorimetric mixing formula:
- m1 is the mass of the liquid you want to add
- m2 is the mass of the liquid that you have
- T1 is the temperature of the liquid you want to add
- T2 is the temperature of the liquid you have
- Tm is the temperature that you want to have after mixing
Suppose you have a 100 ml culture at 39°C and want to shift it to 30°C with the help of 4°C cold fresh medium.
Than the formula is:
That means that m1 is 34.6, so if you add 34.6 ml of 4°C cold fresh medium to your culture it will be 30°C warm.