Template:ASUBME103 f2014 L4: Difference between revisions

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==Protocols==
==Protocol==


'''Materials'''
'''Materials'''
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'''DNA Sample Set-up Procedure'''
'''DNA Sample Set-up Procedure'''
<!-- In your own words, write an easy-to-comprehend list of the steps your group took to set up the PCR reaction in the PCR reaction tubes. End with placing the tubes in to the thermal cycler. Do not copy-paste the instructions from the Workbook. That will be considered plagiarism. -->
# Step 1
# Step 1
# Step 2
# Step 2
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'''PCR Reaction Mix'''
'''OpenPCR program'''
<!-- Make a bulleted list of the components that make up the PCR Reaction Mix -->
<!-- Include a description of the thermal cycling program below. You can use text, a screen capture, a camera snapshot of the computer screen, or a digital drawing (e.g., using shapes and text boxes in Microsoft Powerpoint) -->
* Component 1
 
* Component 2
 
* Component 3...




'''DNA/ primer Mix'''
<!-- Make a bulleted list of the specific components that make up the DNA/ primer Mix -->
* Component 1
* Component 2
* Component 3...




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'''PCR - The Underlying Technology'''<br>
'''PCR - The Underlying Technology'''<br>
<!-- Add a write-up, essay-style, organized into paragraphs with descriptive headers, based on the Q&A's from Section three of your worksheet). BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the PCR video/ tutorial might be useful. Be sure to '''credit the sources''' if you borrow images. -->
<!-- Add a write-up, essay-style, organized into paragraphs with descriptive headers, based on the questions and answers from the Research and Development exercise. BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the PCR video/ tutorial might be useful. Be sure to '''credit the sources''' if you borrow images. You are not allowed to use images from current or past BME 100 students' reports on OpenWetWare. -->




Latest revision as of 12:40, 13 October 2014

BME 100 Fall 2014 Home
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Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3
Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6
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LAB 4 WRITE-UP

Protocol

Materials

  • Item 1
  • Item 2
  • etc.


PCR Reaction Sample List

Tube Label PCR Reaction Sample Patient ID
G# + Positive control none
G# - Negative control none
G# 1-1 Patient 1, replicate 1
G# 1-2 Patient 1, replicate 2
G# 1-3 Patient 1, replicate 3
G# 2-1 Patient 2, replicate 1
G# 2-2 Patient 2, replicate 2
G# 2-3 Patient 2, replicate 3


DNA Sample Set-up Procedure

  1. Step 1
  2. Step 2
  3. Step 3...


OpenPCR program






Research and Development

PCR - The Underlying Technology






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