Template:ASUBME103 f2014 L5: Difference between revisions

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'''SYBR Green Dye'''<br>
'''SYBR Green Dye'''<br>
''[Instructions: A short summary describing SYBR green dye]''<br>
<!-- Instructions: A short summary describing SYBR green dye. -->




'''Single-Drop Fluorimeter'''<br>
'''Single-Drop Fluorimeter'''<br>
''[Instructions: A description of the single-drop fluorimeter device. Add a PHOTO for bonus points]''<br>
<!-- Instructions: A description of the single-drop fluorimeter device. Add a PHOTO for bonus points. -->




'''How the Fluorescence Technique Works'''<br>
'''How the Fluorescence Technique Works'''<br>
''[Instructions: In your own words]''
<!-- Instructions: Write a paragraph about how the fluorescence technique works, in your own words -->




<br>
 
<!-- Note: Be sure to delete the text in brackets: ''[ ]'' -->
<br><br>


==Procedure==
==Procedure==


'''Smart Phone Camera Settings'''<br>
'''Smart Phone Camera Settings'''<br>
''[Instructions: The type of smart phone you used and how you adjusted the camera settings, if applicable (see worksheet page 4).]''
PHONE #1
<!-- Instructions: The type of smart phone you used and how you adjusted the camera settings, if applicable (see worksheet page 4). -->
* Type of Smartphone:
* Type of Smartphone:
** Flash:
** Flash:
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** Saturation:
** Saturation:
** Contrast:
** Contrast:
<br><br>


''[Instructions: If you used an additional phone, describe the other type of smart phone you used and how you adjusted the camera settings, if applicable. If not, delete this part]''
<!-- Instructions: If you used an additional phone, describe the other type of smart phone you used and how you adjusted the camera settings, if applicable. If not, delete the following list. -->
PHONE #2
* Type of Smartphone:
* Type of Smartphone:
** Flash:
** Flash:
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'''Calibration'''<br>
'''Calibration'''<br>
 
<!-- Instructions: Describe how to set up your camera in front of the fluorimeter. Add a PHOTO of this set-up for bonus points. -->
''[Instructions: Describe how to set up your camera in front of the fluorimeter. Add a PHOTO of this set-up for bonus points.]''


* Distance between the smart phone cradle and drop =
* Distance between the smart phone cradle and drop =
''[Instructions: See worksheet page 6.]''




'''Solutions Used for Calibration''' ''[Instructions: See worksheet page 6.]''
'''Solutions Used for Calibration'''
<!-- Refer to the relevant table in your worksheet -->
{| {{table}} width=700
{| {{table}} width=700
|-
|-
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| row 3 cell 1 || row 3 cell 2 || row 3 cell 3 || row 3 cell 4
| row 3 cell 1 || row 3 cell 2 || row 3 cell 3 || row 3 cell 4
|}
|}
''[Add more rows as needed]''
<!-- Add more rows to this table as needed -->




'''Placing Samples onto the Fluorimeter'''
'''Placing Samples onto the Fluorimeter'''
# ''[Instructions: Step one, in your OWN words]''
<!-- Make a numbered list of steps in your own words. The hashtag symbol (Wiki code) will automatically add a number to each list item -->
# ''[Instructions: Step two, in your own words]''
# Step 1
# ''[Instructions: Step three, in your own words]''
# Step 2
# ''[Instructions: Step etc., in your own words]''
# Step 3
# Step 4


<br>
<br>
<!-- Note: Be sure to delete the instruction text in brackets: ''[ ]'' -->
 


==Data Analysis==
==Data Analysis==


'''Representative Images of Samples'''
'''Representative Images of Samples'''
 
<!-- Instructions: (1) Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with no DNA. Instructions: (2) Show an IMAGE <u>where you drew a circle around the droplet</u> with the freehand tool for just one sample '''with''' DNA (positive signal) -->
''[Instructions: Show an IMAGE <u>where you drew a circle around the droplet</u> with the freehand tool for a sample with no DNA]''
 
''[Instructions: Show an IMAGE <u>where you drew a circle around the droplet</u> with the freehand tool for a sample '''with''' DNA (positive signal)]''






'''Image J Values for All Samples'''  
'''Image J Values for All Samples'''  
 
<!-- Instructions: Refer to the appropriate worksheet page. To save time on typing a new Wiki table from scratch, use THIS TOOL to auto-generate a Wiki table: http://excel2wiki.net/wikipedia.php . Copy the headers and values from the Excel spreadsheet you made, paste them into the form field, click submit, copy the Wiki code that the tool generated, and replace TABLE GOES HERE (below) with your auto-generated code.]''
''[Instructions: See worksheet page 8. '''To save time on typing a new Wiki table from scratch''', use THIS TOOL to auto-generate a Wiki table: [http://excel2wiki.net/wikipedia.php Excel-to-Wiki Converter]. Copy the headers and values from the Excel spreadsheet you made, paste them into the form field, click submit, copy the Wiki code that the tool generated, and replace TABLE GOES HERE (below) with your auto-generated code.]''




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'''Fitting a Straight Line'''<br>
'''Fitting a Straight Line'''<br>
 
<!-- Instructions: Place an IMAGE of your Excel plot with a line of best fit here. -->
''[Instructions: Place an IMAGE of your Excel plot with a line of best fit here. See worksheet page 9]''




<br>
<br>
<!-- Note: Be sure to delete the text in brackets: ''[ ]'' -->




<!-- Do not edit below this line -->
<!-- Do not edit below this line -->
|}
|}

Revision as of 14:54, 16 August 2014

BME 100 Fall 2014 Home
People
Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3
Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6
Course Logistics For Instructors
Photos
Wiki Editing Help


OUR TEAM

Name: student
Name: student
Name: student
Name: student
Name: student
Name: student


LAB 5 WRITE-UP

Background Information

SYBR Green Dye


Single-Drop Fluorimeter


How the Fluorescence Technique Works




Procedure

Smart Phone Camera Settings
PHONE #1

  • Type of Smartphone:
    • Flash:
    • ISO setting:
    • White Balance:
    • Exposure:
    • Saturation:
    • Contrast:



PHONE #2

  • Type of Smartphone:
    • Flash:
    • ISO setting:
    • White Balance:
    • Exposure:
    • Saturation:
    • Contrast:


Calibration

  • Distance between the smart phone cradle and drop =


Solutions Used for Calibration

row 1 cell 1 row 1 cell 2 row 1 cell 3 row 1 cell 4
row 2 cell 1 row 2 cell 2 row 2 cell 3 row 2 cell 4
row 3 cell 1 row 3 cell 2 row 3 cell 3 row 3 cell 4


Placing Samples onto the Fluorimeter

  1. Step 1
  2. Step 2
  3. Step 3
  4. Step 4



Data Analysis

Representative Images of Samples


Image J Values for All Samples




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