Template:ASUBME103 f2014 L6

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'''Primer design'''<br>
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'''Primer design and test'''<br>
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<!-- Type-in the sequence of the forward primer and reverse primer in the appropriate spaces -->
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* Disease SNP-specific Forward Primer: Instructions: type the sequence of the forward primer]''
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<u>Non-disease primer pair</u><br>
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* Reverse Primer: ''[Instructions: type the sequence of the reverse primer]''
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<!-- Instructions: Add an IMAGE of your result for testing the NON-DISEASE primer pair from UCSC In-Silico PCR  -->
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How the primers work: ''[Instructions: explain what makes the primers disease-sequence specific. In other words, explain why the primers will amplify DNA that contains the disease-associated SNP, and will not exponentially amplify DNA that has the non-disease allele.]''
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<u>Disease SNP-specific primer pair</u><br>
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<!-- Instructions: Add an IMAGE of your result for testing the DISEASE SNP-SPECIFIC primer pair from UCSC In-Silico PCR  -->
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<u>How the primers work:</u>
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<!-- Instructions: Explain why the Disease SNP-specific primer pair will amplify DNA that contains the disease-associated SNP, and will not exponentially amplify DNA that has the non-disease allele. -->
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==Feature 3: Hardware - PCR Machine & Fluorimeter==
==Feature 3: Hardware - PCR Machine & Fluorimeter==
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<!-- Instructions: Summarize how you will include the PCR machine and fluorimeter in your system. You may add a schematic image. An image is OPTIONAL and will not get bonus points, but it will make your report look really awesome and easy to score. -->
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''[Instructions: Summarize how you will include the PCR machine and fluorimeter in your system. You may add a schematic image. An image is OPTIONAL and will not get bonus points, but it will make your report look really awesome and easy to score.]''
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<!-- Instructions: IF your group has decided to redesign the PCR machine and/or Fluorimeter to address any major weakness(es), explain how in an additional paragraph. -->
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''[Instructions: IF your group has decided to redesign the PCR machine and/or Fluorimeter to address any major weakness(es), explain how in an additional paragraph.]''
 
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<!-- Note: Be sure to delete the text in brackets: ''[ ]'' -->
 
==Bonus Opportunity: What Bayesian Stats Imply About The BME100 Diagnostic Approach==
==Bonus Opportunity: What Bayesian Stats Imply About The BME100 Diagnostic Approach==
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''[Instructions: This section is OPTIONAL, and will get bonus points if answered thoroughly and correctly. Here is a chance to flex some intellectual muscle. In your own words, discuss what the results for calculations 3 and 4 imply about the reliability of PCR for predicting the disease. Please do NOT type the actual numerical values here. Just refer to them as being "close to one" or "very small." The instructors will ask you to submit your actual calculations via a Blackboard quiz. We are doing so for the sake of academic integrity and to curb any temptation to cheat.]''
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<!-- Instructions: This section is OPTIONAL, and will get bonus points if answered thoroughly and correctly. Here is a chance to flex some intellectual muscle. In your own words, discuss what the results for calculations 3 and 4 imply about the reliability of PCR for predicting the disease. Please do NOT type the actual numerical values here. Just refer to them as being "close to one" or "very small." The instructors will ask you to submit your actual calculations via a Blackboard quiz. We are doing so for the sake of academic integrity and to curb any temptation to cheat. -->

Current revision

BME 100 Fall 2014 Home
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Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3
Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6
Course Logistics For Instructors
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Contents

OUR COMPANY

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LAB 6 WRITE-UP

Computer-Aided Design

TinkerCAD


Our Design





Feature 1: Disease SNP-Specific Primers

Background on the disease-associated mutation


Primer design and test

Non-disease primer pair

Disease SNP-specific primer pair

How the primers work:


Feature 2: Consumables Kit

Feature 3: Hardware - PCR Machine & Fluorimeter

Bonus Opportunity: What Bayesian Stats Imply About The BME100 Diagnostic Approach

Personal tools