Template:ASUBME103 f2014 L6: Difference between revisions
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'''Primer design'''<br | '''Primer design and test'''<br> | ||
<u>Non-disease primer pair</u><br> | |||
<!-- Instructions: Add an IMAGE of your result for testing the NON-DISEASE primer pair from UCSC In-Silico PCR --> | |||
How the primers work: | <u>Disease SNP-specific primer pair</u><br> | ||
<!-- Instructions: Add an IMAGE of your result for testing the DISEASE SNP-SPECIFIC primer pair from UCSC In-Silico PCR --> | |||
<u>How the primers work:</u> | |||
<!-- Instructions: Explain why the Disease SNP-specific primer pair will amplify DNA that contains the disease-associated SNP, and will not exponentially amplify DNA that has the non-disease allele. --> | |||
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==Feature 3: Hardware - PCR Machine & Fluorimeter== | ==Feature 3: Hardware - PCR Machine & Fluorimeter== | ||
<!-- Instructions: Summarize how you will include the PCR machine and fluorimeter in your system. You may add a schematic image. An image is OPTIONAL and will not get bonus points, but it will make your report look really awesome and easy to score. --> | |||
<!-- Instructions: IF your group has decided to redesign the PCR machine and/or Fluorimeter to address any major weakness(es), explain how in an additional paragraph. --> | |||
==Bonus Opportunity: What Bayesian Stats Imply About The BME100 Diagnostic Approach== | ==Bonus Opportunity: What Bayesian Stats Imply About The BME100 Diagnostic Approach== | ||
<!-- Instructions: This section is OPTIONAL, and will get bonus points if answered thoroughly and correctly. Here is a chance to flex some intellectual muscle. In your own words, discuss what the results for calculations 3 and 4 imply about the reliability of PCR for predicting the disease. Please do NOT type the actual numerical values here. Just refer to them as being "close to one" or "very small." The instructors will ask you to submit your actual calculations via a Blackboard quiz. We are doing so for the sake of academic integrity and to curb any temptation to cheat. --> | |||
Revision as of 15:48, 16 August 2014
BME 100 Fall 2014 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | ||||||
OUR COMPANY
LAB 6 WRITE-UPComputer-Aided DesignTinkerCAD
Feature 1: Disease SNP-Specific PrimersBackground on the disease-associated mutation
Primer design and test Non-disease primer pair Disease SNP-specific primer pair How the primers work:
Feature 2: Consumables KitFeature 3: Hardware - PCR Machine & FluorimeterBonus Opportunity: What Bayesian Stats Imply About The BME100 Diagnostic Approach |