OUR TEAM
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LAB 5 WRITE-UP
Background Information
SYBR Green Dye
[Instructions: A short summary describing SYBR green dye]
Single-Drop Fluorimeter
[Instructions: A description of the single-drop fluorimeter device. Add a PHOTO for bonus points]
How the Fluorescence Technique Works
[Instructions: In your own words]
Procedure
Smart Phone Camera Settings
- Type of Smartphone:
- Flash:
- ISO setting:
- White Balance:
- Exposure:
- Saturation:
Calibration
[Instructions: Describe how to set up your camera in front of the fluorimeter. Add a PHOTO of this set-up for bonus points.]
- Distance between the smart phone cradle and drop =
[Instructions: See worksheet page 6.]
Solutions Used for Calibration [Instructions: See worksheet page 6.]
row 1 cell 1 |
row 1 cell 2 |
row 1 cell 3 |
row 1 cell 4
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row 2 cell 1 |
row 2 cell 2 |
row 2 cell 3 |
row 2 cell 4
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row 3 cell 1 |
row 3 cell 2 |
row 3 cell 3 |
row 3 cell 4
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[Add more rows as needed]
Placing Samples onto the Fluorimeter
- [Instructions: Step one, in your OWN words]
- [Instructions: Step two, in your own words]
- [Instructions: Step three, in your own words]
- [Instructions: Step etc., in your own words]
Data Analysis
Representative Images of Samples
[Instructions: Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with DNA (positive signal)]
Image J Values for All Samples
TABLE GOES HERE
Calibration curve
PCR Results Summary
- Our positive control PCR result was ____ μg/mL
- Our negative control PCR result was ____ μg/mL
Observed results
- Patient _____ :
- Patient _____ :
Conclusions
- Patient _____ :
- Patient _____ :
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