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A safe and easy way to perform bio-tattoo by DIY tanning was promoted. Dihydroxyacetone (DHA), the most effective additive for sun-free tanning as an alternative to UV tanning, was biosynthesized in suit from glycerol by designed biomolecular machines.
 
The coupling multi-enzyme system, glycerol dehydrogenases (GDH) and NADH oxidase (NOX) enzyme nanoring, catalyzed the biosynthesis of DHA from glycerol with cofactor regeneration. Based on dynamic simulation, site-to-site oriented biomolecular machines was formed by using the affinity of metal ions and cofactor。  Multi-enzyme nanoflowers were formed with the co-precipitated quaternary ammonium and GDH-NOX. The integration brings antibacterial activity and enhances  enzyme activity and stability. The biomolecular machines efficiency varies with  of NADH and glycerol.
 
The coloration levels from light to dark correspond with the DHA percentages. Various patterns and letters can be printed on the skin using packs, providing a safe, painless and easy way for DIY bio-tattoo.
 
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Latest revision as of 03:04, 7 October 2014

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