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(New page: <html><TABLE WIDTH="80%" ><TR ><TD ><A NAME="TBL1" >&nbsp;</A ></TD ></TR ><TR ><TD ALIGN="CENTER" ><B >Table 1. </B ><B >Strains and plasmids used in this work.</B ></TD ></TR ><TR VALIGN...)
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<td ALIGN="CENTER"><B>Table 1. </B><B>Strains and plasmids used in this work.</B></TD></TR>
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<table>
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>Table 1. </B
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<th ALIGN="left" VALIGN="TOP">
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>Strains and plasmids used in this work.</B
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></TD
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<p>Strain or plasmid</TH>
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></TR
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<th ALIGN="left" VALIGN="TOP">
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><TR
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VALIGN="BOTTOM"
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<p>Relevant properties or genotype</TH>
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<th ALIGN="left" VALIGN="TOP"><NOBR>
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<p>Source</NOBR></TH></TR>
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<hr size="1"></TD></TR>
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<tr>
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ALIGN="CENTER"
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<td ALIGN="left" VALIGN="TOP"><NOBR>
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><TABLE
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><TR
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<p>Strains</NOBR></TD>
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><TH
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<td ALIGN="left" VALIGN="TOP"><NOBR></NOBR></TD>
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ALIGN="left"
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<td ALIGN="left" VALIGN="TOP"><NOBR></NOBR></TD></TR>
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VALIGN="TOP"
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<tr>
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><P>Strain or plasmid</TH
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<td ALIGN="left" VALIGN="TOP">
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><TH
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ALIGN="left"
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<p><I>E. coli</I> DH5<IMG SRC="/giflibrary/12/alpha.gif" BORDER="0"></TD>
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VALIGN="TOP"
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<td ALIGN="left" VALIGN="TOP">
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><P>Relevant properties or genotype</TH
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><TH
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<p>F<SUP>-</SUP>, <I><IMG SRC="/giflibrary/12/phgr.gif" BORDER="0" ALIGN="ABSBOTTOM"></I>80d, <I>lacZ</I><IMG SRC="/giflibrary/12/Delta.gif" BORDER="0">M15, <I>endA</I>1, <I>recA</I>1, <I>hsdR</I>17(r<SUB>K</SUB><SUP>-</SUP>m<SUB>K</SUB><SUP>-</SUP>), <I>supE</I>44, <I>thi</I>-1, <I>gyrA</I>96, <I>relA</I>1, <IMG SRC="/giflibrary/12/Delta.gif" BORDER="0">(<I>lacZYA-argF</I>)U169</TD>
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><NOBR
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<p>[a]</NOBR></TD></TR>
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><P>Source</NOBR
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<tr>
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<p><I>E. coli</I> M15 [pREP4]</TD>
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><TD
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<td ALIGN="left" VALIGN="TOP">
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COLSPAN="3"
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><HR
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<p>F<SUP>-</SUP>, <I>lacZ<IMG SRC="/giflibrary/12/Delta.gif" BORDER="0">M15, thi</I>-1, <I>lac<SUP>-</SUP></I>, <I>mtl<SUP>-</SUP></I>, <I>recA<SUP>+</SUP></I> , Km<SUP>R</SUP></TD>
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size="1"></TD
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<td ALIGN="left" VALIGN="TOP"><NOBR>
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></TR
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><TR
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<p>[a]</NOBR></TD></TR>
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><TD
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<tr>
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ALIGN="left"
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<td ALIGN="left" VALIGN="TOP">
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VALIGN="TOP"
+
 
-
><NOBR
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<p><I>Pseudomonas putida</I> KT2440</TD>
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><P>Strains</NOBR
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<td ALIGN="left" VALIGN="TOP">
-
></TD
+
 
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><TD
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<p>type strain</TD>
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ALIGN="left"
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<td ALIGN="left" VALIGN="TOP"><NOBR>
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VALIGN="TOP"
+
 
-
><NOBR
+
<p>[b]</NOBR></TD></TR>
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></NOBR
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<tr>
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></TD
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<td ALIGN="left" VALIGN="TOP">
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><TD
+
 
-
ALIGN="left"
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<p><I>Synechocystis</I> sp. PCC 6803</TD>
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VALIGN="TOP"
+
<td ALIGN="left" VALIGN="TOP">
-
><NOBR
+
 
-
></NOBR
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<p>type strain</TD>
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></TD
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<td ALIGN="left" VALIGN="TOP"><NOBR>
-
></TR
+
 
-
><TR
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<p>[c]</NOBR></TD></TR>
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><TD
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<tr>
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ALIGN="left"
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<td ALIGN="left" VALIGN="TOP"><NOBR>
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VALIGN="TOP"
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><P><I
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<p>Plasmids</NOBR></TD>
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>E. coli</I
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<td ALIGN="left" VALIGN="TOP"><NOBR></NOBR></TD>
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> DH5<IMG
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<td ALIGN="left" VALIGN="TOP"><NOBR></NOBR></TD></TR>
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SRC="/giflibrary/12/alpha.gif"
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<tr>
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BORDER="0"></TD
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<td ALIGN="left" VALIGN="TOP"><NOBR>
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><TD
+
 
-
ALIGN="left"
+
<p>pCAR16</NOBR></TD>
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VALIGN="TOP"
+
<td ALIGN="left" VALIGN="TOP">
-
><P>F<SUP
+
 
-
>-</SUP
+
<p><IMG SRC="/giflibrary/12/beta.gif" BORDER="0" ALIGN="ABSBOTTOM">-carotene biosynthesis gene cluster from <I>Pantoea ananatis</I> in pUC19, Amp<SUP>R</SUP></TD>
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>, <I
+
<td ALIGN="left" VALIGN="TOP"><NOBR>
-
><IMG
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-
SRC="/giflibrary/12/phgr.gif"
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<p>[<A HREF="#BIB27">27</A>]</NOBR></TD></TR>
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BORDER="0"
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<tr>
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ALIGN="ABSBOTTOM"></I
+
<td ALIGN="left" VALIGN="TOP"><NOBR>
-
>80d, <I
+
 
-
>lacZ</I
+
<p>pJF119EH</NOBR></TD>
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><IMG
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<td ALIGN="left" VALIGN="TOP">
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SRC="/giflibrary/12/Delta.gif"
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-
BORDER="0">M15, <I
+
<p>cloning vector, tac-promoter, IPTG inducible, Amp<SUP>R</SUP></TD>
-
>endA</I
+
<td ALIGN="left" VALIGN="TOP"><NOBR>
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>1, <I
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-
>recA</I
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<p>[<A HREF="#BIB25">25</A>]</NOBR></TD></TR>
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>1, <I
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<tr>
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>hsdR</I
+
<td ALIGN="left" VALIGN="TOP"><NOBR>
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>17(r<SUB
+
 
-
>K</SUB
+
<p>pJF119<IMG SRC="/giflibrary/12/Delta.gif" BORDER="0">N</NOBR></TD>
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><SUP
+
<td ALIGN="left" VALIGN="TOP">
-
>-</SUP
+
 
-
>m<SUB
+
<p>cloning vector, RBS, tac-promoter, IPTG inducible, Amp<SUP>R</SUP></TD>
-
>K</SUB
+
<td ALIGN="left" VALIGN="TOP">
-
><SUP
+
 
-
>-</SUP
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<p>this study</TD></TR>
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>), <I
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<tr>
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>supE</I
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<td ALIGN="left" VALIGN="TOP"><NOBR>
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>44, <I
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-
>thi</I
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<p>pAW229</NOBR></TD>
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>-1, <I
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<td ALIGN="left" VALIGN="TOP">
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>gyrA</I
+
 
-
>96, <I
+
<p>cloning vector, RBS, <I>rha</I>-promoter, <FONT SIZE="-1">L</FONT>-rhamnose inducible, Cm<SUP>R</SUP></TD>
-
>relA</I
+
<td ALIGN="left" VALIGN="TOP"><NOBR>
-
>1, <IMG
+
 
-
SRC="/giflibrary/12/Delta.gif"
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<p>[<A HREF="#BIB26">26</A>]</NOBR></TD></TR>
-
BORDER="0">(<I
+
<tr>
-
>lacZYA-argF</I
+
<td ALIGN="left" VALIGN="TOP"><NOBR>
-
>)U169</TD
+
 
-
><TD
+
<p>pQE31-VTE1</NOBR></TD>
-
ALIGN="left"
+
<td ALIGN="left" VALIGN="TOP">
-
VALIGN="TOP"
+
 
-
><NOBR
+
<p>tocopherol-cyclase (vte1) from <I>Arabidopsis thaliana</I> in pQE31, IPTG inducible, Amp<SUP>R</SUP></TD>
-
><P>[a]</NOBR
+
<td ALIGN="left" VALIGN="TOP"><NOBR>
-
></TD
+
 
-
></TR
+
<p>[<A HREF="#BIB18A">18a</A>]</NOBR></TD></TR>
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><TR
+
<tr>
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><TD
+
<td ALIGN="left" VALIGN="TOP"><NOBR>
-
ALIGN="left"
+
 
-
VALIGN="TOP"
+
<p>pCAS2JF</NOBR></TD>
-
><P><I
+
<td ALIGN="left" VALIGN="TOP">
-
>E. coli</I
+
 
-
> M15 [pREP4]</TD
+
<p>1.2 kb EcoRI/BamHI PCR fragment <I>hpd</I> in pJF119EH</TD>
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><TD
+
<td ALIGN="left" VALIGN="TOP">
-
ALIGN="left"
+
 
-
VALIGN="TOP"
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<p>this study</TD></TR>
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><P>F<SUP
+
<tr>
-
>-</SUP
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<td ALIGN="left" VALIGN="TOP"><NOBR>
-
>, <I
+
 
-
>lacZ<IMG
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<p>pCAS8</NOBR></TD>
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SRC="/giflibrary/12/Delta.gif"
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<td ALIGN="left" VALIGN="TOP">
-
BORDER="0">M15, thi</I
+
 
-
>-1, <I
+
<p>1.1 kb NdeI/BamHI PCR fragment <I>ggh</I> in pJF119<IMG SRC="/giflibrary/12/Delta.gif" BORDER="0">N</TD>
-
>lac<SUP
+
<td ALIGN="left" VALIGN="TOP">
-
>-</SUP
+
 
-
></I
+
<p>this study</TD></TR>
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>, <I
+
<tr>
-
>mtl<SUP
+
<td ALIGN="left" VALIGN="TOP"><NOBR>
-
>-</SUP
+
 
-
></I
+
<p>pCAS11</NOBR></TD>
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>, <I
+
<td ALIGN="left" VALIGN="TOP">
-
>recA<SUP
+
 
-
>+</SUP
+
<p>0.9 kb BglII/BamHI PCR fragment <I>crtE</I> in pCAS8</TD>
-
></I
+
<td ALIGN="left" VALIGN="TOP">
-
> , Km<SUP
+
 
-
>R</SUP
+
<p>this study</TD></TR>
-
></TD
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<tr>
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><TD
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<td ALIGN="left" VALIGN="TOP"><NOBR>
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ALIGN="left"
+
 
-
VALIGN="TOP"
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<p>pCAS12</NOBR></TD>
-
><NOBR
+
<td ALIGN="left" VALIGN="TOP">
-
><P>[a]</NOBR
+
 
-
></TD
+
<p>1.0 kb BglII/BamHI PCR fragment <I>hpt</I> in pCAS11</TD>
-
></TR
+
<td ALIGN="left" VALIGN="TOP">
-
><TR
+
 
-
><TD
+
<p>this study</TD></TR>
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ALIGN="left"
+
<tr>
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VALIGN="TOP"
+
<td ALIGN="left" VALIGN="TOP"><NOBR>
-
><P><I
+
 
-
>Pseudomonas putida</I
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<p>pCAS15</NOBR></TD>
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> KT2440</TD
+
<td ALIGN="left" VALIGN="TOP">
-
><TD
+
 
-
ALIGN="left"
+
<p>1.2 kb NdeI/BamHI PCR fragment <I>hpd</I> in pAW229</TD>
-
VALIGN="TOP"
+
<td ALIGN="left" VALIGN="TOP">
-
><P>type strain</TD
+
 
-
><TD
+
<p>this study</TD></TR>
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ALIGN="left"
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<tr>
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VALIGN="TOP"
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<td ALIGN="left" VALIGN="TOP"><NOBR>
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><NOBR
+
 
-
><P>[b]</NOBR
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<p>pCAS18</NOBR></TD>
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></TD
+
<td ALIGN="left" VALIGN="TOP">
-
></TR
+
 
-
><TR
+
<p>1.2 kb BglII/BamHI PCR fragment <I>hpd</I> in pCAS11</TD>
-
><TD
+
<td ALIGN="left" VALIGN="TOP">
-
ALIGN="left"
+
 
-
VALIGN="TOP"
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<p>this study</TD></TR>
-
><P><I
+
<tr>
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>Synechocystis</I
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<td ALIGN="left" VALIGN="TOP"><NOBR>
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> sp. PCC 6803</TD
+
 
-
><TD
+
<p>pCAS19</NOBR></TD>
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ALIGN="left"
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<td ALIGN="left" VALIGN="TOP">
-
VALIGN="TOP"
+
 
-
><P>type strain</TD
+
<p>1.0 kb NdeI/BamHI PCR fragment <I>hpt</I> in pAW229</TD>
-
><TD
+
<td ALIGN="left" VALIGN="TOP">
-
ALIGN="left"
+
 
-
VALIGN="TOP"
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<p>this study</TD></TR>
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><NOBR
+
<tr>
-
><P>[c]</NOBR
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<td ALIGN="left" VALIGN="TOP"><NOBR>
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></TD
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-
></TR
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<p>pCAS23</NOBR></TD>
-
><TR
+
<td ALIGN="left" VALIGN="TOP">
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><TD
+
 
-
ALIGN="left"
+
<p>1.0 kb NdeI/BamHI PCR fragment <I>hpt</I> in pJF119<IMG SRC="/giflibrary/12/Delta.gif" BORDER="0">N</TD>
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VALIGN="TOP"
+
<td ALIGN="left" VALIGN="TOP">
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><NOBR
+
 
-
><P>Plasmids</NOBR
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<p>this study</TD></TR>
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></TD
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<tr>
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><TD
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<td ALIGN="left" VALIGN="TOP"><NOBR>
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ALIGN="left"
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VALIGN="TOP"
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<p>pCAS24</NOBR></TD>
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><NOBR
+
<td ALIGN="left" VALIGN="TOP">
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></NOBR
+
 
-
></TD
+
<p>0.9 kb NdeI/BamHI PCR fragment <I>crtE</I> in pCAS23</TD>
-
><TD
+
<td ALIGN="left" VALIGN="TOP">
-
ALIGN="left"
+
 
-
VALIGN="TOP"
+
<p>this study</TD></TR>
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><NOBR
+
<tr>
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></NOBR
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<td ALIGN="left" VALIGN="TOP"><NOBR>
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></TD
+
 
-
></TR
+
<p>pCAS27</NOBR></TD>
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><TR
+
<td ALIGN="left" VALIGN="TOP">
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><TD
+
 
-
ALIGN="left"
+
<p>1.1 kb NdeI/BamHI PCR fragment <I>cyc-sy</I> in pAW229</TD>
-
VALIGN="TOP"
+
<td ALIGN="left" VALIGN="TOP">
-
><NOBR
+
 
-
><P>pCAR16</NOBR
+
<p>this study</TD></TR>
-
></TD
+
<tr>
-
><TD
+
<td ALIGN="left" VALIGN="TOP"><NOBR>
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ALIGN="left"
+
 
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VALIGN="TOP"
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<p>pCAS29</NOBR></TD>
-
><P><IMG
+
<td ALIGN="left" VALIGN="TOP">
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SRC="/giflibrary/12/beta.gif"
+
 
-
BORDER="0"
+
<p>1.2 kb BglII/BamHI PCR fragment <I>hpd</I> in pCAS18</TD>
-
ALIGN="ABSBOTTOM">-carotene biosynthesis gene cluster from <I
+
<td ALIGN="left" VALIGN="TOP">
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>Pantoea ananatis</I
+
 
-
> in pUC19, Amp<SUP
+
<p>this study</TD></TR>
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>R</SUP
+
<tr>
-
></TD
+
<td ALIGN="left" VALIGN="TOP"><NOBR>
-
><TD
+
 
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ALIGN="left"
+
<p>pCAS30</NOBR></TD>
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VALIGN="TOP"
+
<td ALIGN="left" VALIGN="TOP">
-
><NOBR
+
 
-
><P>[<A
+
<p>0.9 kb NdeI/BamHI PCR fragment <I>crtE</I> in pJF119<IMG SRC="/giflibrary/12/Delta.gif" BORDER="0">N</TD>
-
HREF="#BIB27"
+
<td ALIGN="left" VALIGN="TOP">
-
>27</A
+
 
-
>]</NOBR
+
<p>this study</TD></TR>
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></TD
+
<tr>
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></TR
+
<td ALIGN="left" VALIGN="TOP"><NOBR>
-
><TR
+
 
-
><TD
+
<p>pCAS37</NOBR></TD>
-
ALIGN="left"
+
<td ALIGN="left" VALIGN="TOP">
-
VALIGN="TOP"
+
 
-
><NOBR
+
<p>1.2 kb BglII/BamHI PCR fragment <I>hpd</I> in pCAS24</TD>
-
><P>pJF119EH</NOBR
+
<td ALIGN="left" VALIGN="TOP">
-
></TD
+
 
-
><TD
+
<p>this study</TD></TR>
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ALIGN="left"
+
<tr>
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VALIGN="TOP"
+
<td ALIGN="left" VALIGN="TOP"><NOBR>
-
><P>cloning vector, tac-promoter, IPTG inducible, Amp<SUP
+
 
-
>R</SUP
+
<p>pCAS47</NOBR></TD>
-
></TD
+
<td ALIGN="left" VALIGN="TOP">
-
><TD
+
 
-
ALIGN="left"
+
<p>1.3 kb BglII PCR fragment <I>vte1</I> in pCAS29</TD>
-
VALIGN="TOP"
+
<td ALIGN="left" VALIGN="TOP">
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><NOBR
+
 
-
><P>[<A
+
<p>this study</TD></TR></TABLE></TD></TR>
-
HREF="#BIB25"
+
<tr VALIGN="BOTTOM">
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>25</A
+
<td>
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>]</NOBR
+
<hr size="2" noshade="1"></TD></TR></TABLE></CENTER><CENTER>
-
></TD
+
<table WIDTH="80%">
-
></TR
+
<tr>
-
><TR
+
<td><FONT SIZE="-1">&nbsp;&nbsp;&nbsp;[a] Commercial strain. [b] Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ). [c] A kind gift from Prof. Forchhammer, Universit&auml;t-Gie&szlig;en (Germany).
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><TD
+
<br></FONT></TD></TR></TABLE>
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ALIGN="left"
+
</html>
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VALIGN="TOP"
+
-
><NOBR
+
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><P>pJF119<IMG
+
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SRC="/giflibrary/12/Delta.gif"
+
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BORDER="0">N</NOBR
+
-
></TD
+
-
><TD
+
-
ALIGN="left"
+
-
VALIGN="TOP"
+
-
><P>cloning vector, RBS, tac-promoter, IPTG inducible, Amp<SUP
+
-
>R</SUP
+
-
></TD
+
-
><TD
+
-
ALIGN="left"
+
-
VALIGN="TOP"
+
-
><P>this study</TD
+
-
></TR
+
-
><TR
+
-
><TD
+
-
ALIGN="left"
+
-
VALIGN="TOP"
+
-
><NOBR
+
-
><P>pAW229</NOBR
+
-
></TD
+
-
><TD
+
-
ALIGN="left"
+
-
VALIGN="TOP"
+
-
><P>cloning vector, RBS, <I
+
-
>rha</I
+
-
>-promoter, <FONT
+
-
SIZE="-1"
+
-
>L</FONT
+
-
>-rhamnose inducible, Cm<SUP
+
-
>R</SUP
+
-
></TD
+
-
><TD
+
-
ALIGN="left"
+
-
VALIGN="TOP"
+
-
><NOBR
+
-
><P>[<A
+
-
HREF="#BIB26"
+
-
>26</A
+
-
>]</NOBR
+
-
></TD
+
-
></TR
+
-
><TR
+
-
><TD
+
-
ALIGN="left"
+
-
VALIGN="TOP"
+
-
><NOBR
+
-
><P>pQE31-VTE1</NOBR
+
-
></TD
+
-
><TD
+
-
ALIGN="left"
+
-
VALIGN="TOP"
+
-
><P>tocopherol-cyclase (vte1) from <I
+
-
>Arabidopsis thaliana</I
+
-
> in pQE31, IPTG inducible, Amp<SUP
+
-
>R</SUP
+
-
></TD
+
-
><TD
+
-
ALIGN="left"
+
-
VALIGN="TOP"
+
-
><NOBR
+
-
><P>[<A
+
-
HREF="#BIB18A"
+
-
>18a</A
+
-
>]</NOBR
+
-
></TD
+
-
></TR
+
-
><TR
+
-
><TD
+
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ALIGN="left"
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VALIGN="TOP"
+
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><NOBR
+
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><P>pCAS2JF</NOBR
+
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></TD
+
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><TD
+
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ALIGN="left"
+
-
VALIGN="TOP"
+
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><P>1.2 kb EcoRI/BamHI PCR fragment <I
+
-
>hpd</I
+
-
> in pJF119EH</TD
+
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><TD
+
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ALIGN="left"
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VALIGN="TOP"
+
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><P>this study</TD
+
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></TR
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><TR
+
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><TD
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ALIGN="left"
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VALIGN="TOP"
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><NOBR
+
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><P>pCAS8</NOBR
+
-
></TD
+
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><TD
+
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ALIGN="left"
+
-
VALIGN="TOP"
+
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><P>1.1 kb NdeI/BamHI PCR fragment <I
+
-
>ggh</I
+
-
> in pJF119<IMG
+
-
SRC="/giflibrary/12/Delta.gif"
+
-
BORDER="0">N</TD
+
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><TD
+
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ALIGN="left"
+
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VALIGN="TOP"
+
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><P>this study</TD
+
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></TR
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><TR
+
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><TD
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VALIGN="TOP"
+
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><NOBR
+
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><P>pCAS11</NOBR
+
-
></TD
+
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><TD
+
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ALIGN="left"
+
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VALIGN="TOP"
+
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><P>0.9 kb BglII/BamHI PCR fragment <I
+
-
>crtE</I
+
-
> in pCAS8</TD
+
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><TD
+
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ALIGN="left"
+
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VALIGN="TOP"
+
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><P>this study</TD
+
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></TR
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><TR
+
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><TD
+
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ALIGN="left"
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VALIGN="TOP"
+
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><NOBR
+
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><P>pCAS12</NOBR
+
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></TD
+
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><TD
+
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ALIGN="left"
+
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VALIGN="TOP"
+
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><P>1.0 kb BglII/BamHI PCR fragment <I
+
-
>hpt</I
+
-
> in pCAS11</TD
+
-
><TD
+
-
ALIGN="left"
+
-
VALIGN="TOP"
+
-
><P>this study</TD
+
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></TR
+
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><TR
+
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><TD
+
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ALIGN="left"
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VALIGN="TOP"
+
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><NOBR
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><P>pCAS15</NOBR
+
-
></TD
+
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><TD
+
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ALIGN="left"
+
-
VALIGN="TOP"
+
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><P>1.2 kb NdeI/BamHI PCR fragment <I
+
-
>hpd</I
+
-
> in pAW229</TD
+
-
><TD
+
-
ALIGN="left"
+
-
VALIGN="TOP"
+
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><P>this study</TD
+
-
></TR
+
-
><TR
+
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><TD
+
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ALIGN="left"
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VALIGN="TOP"
+
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><NOBR
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><P>pCAS18</NOBR
+
-
></TD
+
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><TD
+
-
ALIGN="left"
+
-
VALIGN="TOP"
+
-
><P>1.2 kb BglII/BamHI PCR fragment <I
+
-
>hpd</I
+
-
> in pCAS11</TD
+
-
><TD
+
-
ALIGN="left"
+
-
VALIGN="TOP"
+
-
><P>this study</TD
+
-
></TR
+
-
><TR
+
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><TD
+
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ALIGN="left"
+
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VALIGN="TOP"
+
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><NOBR
+
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><P>pCAS19</NOBR
+
-
></TD
+
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><TD
+
-
ALIGN="left"
+
-
VALIGN="TOP"
+
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><P>1.0 kb NdeI/BamHI PCR fragment <I
+
-
>hpt</I
+
-
> in pAW229</TD
+
-
><TD
+
-
ALIGN="left"
+
-
VALIGN="TOP"
+
-
><P>this study</TD
+
-
></TR
+
-
><TR
+
-
><TD
+
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ALIGN="left"
+
-
VALIGN="TOP"
+
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><NOBR
+
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><P>pCAS23</NOBR
+
-
></TD
+
-
><TD
+
-
ALIGN="left"
+
-
VALIGN="TOP"
+
-
><P>1.0 kb NdeI/BamHI PCR fragment <I
+
-
>hpt</I
+
-
> in pJF119<IMG
+
-
SRC="/giflibrary/12/Delta.gif"
+
-
BORDER="0">N</TD
+
-
><TD
+
-
ALIGN="left"
+
-
VALIGN="TOP"
+
-
><P>this study</TD
+
-
></TR
+
-
><TR
+
-
><TD
+
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ALIGN="left"
+
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VALIGN="TOP"
+
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><NOBR
+
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><P>pCAS24</NOBR
+
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></TD
+
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><TD
+
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ALIGN="left"
+
-
VALIGN="TOP"
+
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><P>0.9 kb NdeI/BamHI PCR fragment <I
+
-
>crtE</I
+
-
> in pCAS23</TD
+
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><TD
+
-
ALIGN="left"
+
-
VALIGN="TOP"
+
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><P>this study</TD
+
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></TR
+
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><TR
+
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><TD
+
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ALIGN="left"
+
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VALIGN="TOP"
+
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><NOBR
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><P>pCAS27</NOBR
+
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></TD
+
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><TD
+
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ALIGN="left"
+
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VALIGN="TOP"
+
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><P>1.1 kb NdeI/BamHI PCR fragment <I
+
-
>cyc-sy</I
+
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> in pAW229</TD
+
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><TD
+
-
ALIGN="left"
+
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VALIGN="TOP"
+
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><P>this study</TD
+
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></TR
+
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><TR
+
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><TD
+
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+
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VALIGN="TOP"
+
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><NOBR
+
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><P>pCAS29</NOBR
+
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></TD
+
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><TD
+
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ALIGN="left"
+
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VALIGN="TOP"
+
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><P>1.2 kb BglII/BamHI PCR fragment <I
+
-
>hpd</I
+
-
> in pCAS18</TD
+
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><TD
+
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ALIGN="left"
+
-
VALIGN="TOP"
+
-
><P>this study</TD
+
-
></TR
+
-
><TR
+
-
><TD
+
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ALIGN="left"
+
-
VALIGN="TOP"
+
-
><NOBR
+
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><P>pCAS30</NOBR
+
-
></TD
+
-
><TD
+
-
ALIGN="left"
+
-
VALIGN="TOP"
+
-
><P>0.9 kb NdeI/BamHI PCR fragment <I
+
-
>crtE</I
+
-
> in pJF119<IMG
+
-
SRC="/giflibrary/12/Delta.gif"
+
-
BORDER="0">N</TD
+
-
><TD
+
-
ALIGN="left"
+
-
VALIGN="TOP"
+
-
><P>this study</TD
+
-
></TR
+
-
><TR
+
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><TD
+
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ALIGN="left"
+
-
VALIGN="TOP"
+
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><NOBR
+
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><P>pCAS37</NOBR
+
-
></TD
+
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><TD
+
-
ALIGN="left"
+
-
VALIGN="TOP"
+
-
><P>1.2 kb BglII/BamHI PCR fragment <I
+
-
>hpd</I
+
-
> in pCAS24</TD
+
-
><TD
+
-
ALIGN="left"
+
-
VALIGN="TOP"
+
-
><P>this study</TD
+
-
></TR
+
-
><TR
+
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><TD
+
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ALIGN="left"
+
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+
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><NOBR
+
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><P>pCAS47</NOBR
+
-
></TD
+
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><TD
+
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ALIGN="left"
+
-
VALIGN="TOP"
+
-
><P>1.3 kb BglII PCR fragment <I
+
-
>vte1</I
+
-
> in pCAS29</TD
+
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><TD
+
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ALIGN="left"
+
-
VALIGN="TOP"
+
-
><P>this study</TD
+
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></TR
+
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>&nbsp;&nbsp;&nbsp;[a] Commercial strain. [b] Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ). [c] A kind gift from Prof. Forchhammer, Universit&auml;t-Gie&szlig;en (Germany).<BR></FONT
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Revision as of 21:02, 19 July 2009

 
Table 1. Strains and plasmids used in this work.

Strain or plasmid

Relevant properties or genotype

Source


Strains

E. coli DH5

F-, 80d, lacZM15, endA1, recA1, hsdR17(rK-mK-), supE44, thi-1, gyrA96, relA1, (lacZYA-argF)U169

[a]

E. coli M15 [pREP4]

F-, lacZM15, thi-1, lac-, mtl-, recA+ , KmR

[a]

Pseudomonas putida KT2440

type strain

[b]

Synechocystis sp. PCC 6803

type strain

[c]

Plasmids

pCAR16

-carotene biosynthesis gene cluster from Pantoea ananatis in pUC19, AmpR

[27]

pJF119EH

cloning vector, tac-promoter, IPTG inducible, AmpR

[25]

pJF119N

cloning vector, RBS, tac-promoter, IPTG inducible, AmpR

this study

pAW229

cloning vector, RBS, rha-promoter, L-rhamnose inducible, CmR

[26]

pQE31-VTE1

tocopherol-cyclase (vte1) from Arabidopsis thaliana in pQE31, IPTG inducible, AmpR

[18a]

pCAS2JF

1.2 kb EcoRI/BamHI PCR fragment hpd in pJF119EH

this study

pCAS8

1.1 kb NdeI/BamHI PCR fragment ggh in pJF119N

this study

pCAS11

0.9 kb BglII/BamHI PCR fragment crtE in pCAS8

this study

pCAS12

1.0 kb BglII/BamHI PCR fragment hpt in pCAS11

this study

pCAS15

1.2 kb NdeI/BamHI PCR fragment hpd in pAW229

this study

pCAS18

1.2 kb BglII/BamHI PCR fragment hpd in pCAS11

this study

pCAS19

1.0 kb NdeI/BamHI PCR fragment hpt in pAW229

this study

pCAS23

1.0 kb NdeI/BamHI PCR fragment hpt in pJF119N

this study

pCAS24

0.9 kb NdeI/BamHI PCR fragment crtE in pCAS23

this study

pCAS27

1.1 kb NdeI/BamHI PCR fragment cyc-sy in pAW229

this study

pCAS29

1.2 kb BglII/BamHI PCR fragment hpd in pCAS18

this study

pCAS30

0.9 kb NdeI/BamHI PCR fragment crtE in pJF119N

this study

pCAS37

1.2 kb BglII/BamHI PCR fragment hpd in pCAS24

this study

pCAS47

1.3 kb BglII PCR fragment vte1 in pCAS29

this study


   [a] Commercial strain. [b] Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ). [c] A kind gift from Prof. Forchhammer, Universität-Gießen (Germany).

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