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Table 1. Strains and plasmids used in this work.

Strain or plasmid	Relevant properties or genotype	Source

Strains
E. coli DH5	F^-, 80d, lacZM15, endA1, recA1, hsdR17(r_K^-m_K^-), supE44, thi-1, gyrA96, relA1, (lacZYA-argF)U169	[a]
E. coli M15 [pREP4]	F^-, lacZM15, thi-1, lac^-, mtl^-, recA⁺ , Km^R	[a]
Pseudomonas putida KT2440	type strain	[b]
Synechocystis sp. PCC 6803	type strain	[c]
Plasmids
pCAR16	-carotene biosynthesis gene cluster from Pantoea ananatis in pUC19, Amp^R	[27]
pJF119EH	cloning vector, tac-promoter, IPTG inducible, Amp^R	[25]
pJF119N	cloning vector, RBS, tac-promoter, IPTG inducible, Amp^R	this study
pAW229	cloning vector, RBS, rha-promoter, L-rhamnose inducible, Cm^R	[26]
pQE31-VTE1	tocopherol-cyclase (vte1) from Arabidopsis thaliana in pQE31, IPTG inducible, Amp^R	[18a]
pCAS2JF	1.2 kb EcoRI/BamHI PCR fragment hpd in pJF119EH	this study
pCAS8	1.1 kb NdeI/BamHI PCR fragment ggh in pJF119N	this study
pCAS11	0.9 kb BglII/BamHI PCR fragment crtE in pCAS8	this study
pCAS12	1.0 kb BglII/BamHI PCR fragment hpt in pCAS11	this study
pCAS15	1.2 kb NdeI/BamHI PCR fragment hpd in pAW229	this study
pCAS18	1.2 kb BglII/BamHI PCR fragment hpd in pCAS11	this study
pCAS19	1.0 kb NdeI/BamHI PCR fragment hpt in pAW229	this study
pCAS23	1.0 kb NdeI/BamHI PCR fragment hpt in pJF119N	this study
pCAS24	0.9 kb NdeI/BamHI PCR fragment crtE in pCAS23	this study
pCAS27	1.1 kb NdeI/BamHI PCR fragment cyc-sy in pAW229	this study
pCAS29	1.2 kb BglII/BamHI PCR fragment hpd in pCAS18	this study
pCAS30	0.9 kb NdeI/BamHI PCR fragment crtE in pJF119N	this study
pCAS37	1.2 kb BglII/BamHI PCR fragment hpd in pCAS24	this study
pCAS47	1.3 kb BglII PCR fragment vte1 in pCAS29	this study

[a] Commercial strain. [b] Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ). [c] A kind gift from Prof. Forchhammer, Universität-Gießen (Germany).

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->&nbsp;</A
+<td ALIGN="CENTER"><B>Table 1. </B><B>Strains and plasmids used in this work.</B></TD></TR>
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->Strains and plasmids used in this work.</B
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-><P>Relevant properties or genotype</TH
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-><NOBR
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->, <I
+<td ALIGN="left" VALIGN="TOP"><NOBR>
-><IMG
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+<p>[<A HREF="#BIB27">27</A>]</NOBR></TD></TR>
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-ALIGN="left"
-VALIGN="TOP"
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-><TD
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-><NOBR
-><P>Plasmids</NOBR
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-VALIGN="TOP"
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-></NOBR
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+<td ALIGN="left" VALIGN="TOP">
-ALIGN="left"
-VALIGN="TOP"
+<p>this study</TD></TR>
-><NOBR
+<tr>
-></NOBR
+<td ALIGN="left" VALIGN="TOP"><NOBR>
-></TD
-></TR
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-><TD
-ALIGN="left"
+<p>1.1 kb NdeI/BamHI PCR fragment <I>cyc-sy</I> in pAW229</TD>
-VALIGN="TOP"
+<td ALIGN="left" VALIGN="TOP">
-><NOBR
-><P>pCAR16</NOBR
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-ALIGN="left"
-VALIGN="TOP"
+<p>pCAS29</NOBR></TD>
-><P><IMG
+<td ALIGN="left" VALIGN="TOP">
-SRC="/giflibrary/12/beta.gif"
-BORDER="0"
+<p>1.2 kb BglII/BamHI PCR fragment <I>hpd</I> in pCAS18</TD>
-ALIGN="ABSBOTTOM">-carotene biosynthesis gene cluster from <I
+<td ALIGN="left" VALIGN="TOP">
->Pantoea ananatis</I
-> in pUC19, Amp<SUP
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+<p>pCAS30</NOBR></TD>
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-><NOBR
-><P>[<A
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-HREF="#BIB27"
+<td ALIGN="left" VALIGN="TOP">
->27</A
->]</NOBR
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-></TD
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-></TR
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-><TR
-><TD
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-VALIGN="TOP"
-><NOBR
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-><P>pJF119EH</NOBR
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-></TD
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+<td ALIGN="left" VALIGN="TOP"><NOBR>
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->R</SUP
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-><TD
-ALIGN="left"
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-VALIGN="TOP"
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-><NOBR
-><P>[<A
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->25</A
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->]</NOBR
+<hr size="2" noshade="1"></TD></TR></TABLE></CENTER><CENTER>
-></TD
+<table WIDTH="80%">
-></TR
+<tr>
-><TR
+<td><FONT SIZE="-1">&nbsp;&nbsp;&nbsp;[a] Commercial strain. [b] Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ). [c] A kind gift from Prof. Forchhammer, Universit&auml;t-Gie&szlig;en (Germany).
-><TD
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+</html>
-VALIGN="TOP"
-><NOBR
-><P>pJF119<IMG
-SRC="/giflibrary/12/Delta.gif"
-BORDER="0">N</NOBR
-></TD
-><TD
-ALIGN="left"
-VALIGN="TOP"
-><P>cloning vector, RBS, tac-promoter, IPTG inducible, Amp<SUP
->R</SUP
-></TD
-><TD
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-VALIGN="TOP"
-><P>this study</TD
-></TR
-><TR
-><TD
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-><NOBR
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-><TD
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-><P>cloning vector, RBS, <I
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->-promoter, <FONT
-SIZE="-1"
->L</FONT
->-rhamnose inducible, Cm<SUP
->R</SUP
-></TD
-><TD
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->26</A
->]</NOBR
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-><TR
-><TD
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-VALIGN="TOP"
-><NOBR
-><P>pQE31-VTE1</NOBR
-></TD
-><TD
-ALIGN="left"
-VALIGN="TOP"
-><P>tocopherol-cyclase (vte1) from <I
->Arabidopsis thaliana</I
-> in pQE31, IPTG inducible, Amp<SUP
->R</SUP
-></TD
-><TD
-ALIGN="left"
-VALIGN="TOP"
-><NOBR
-><P>[<A
-HREF="#BIB18A"
->18a</A
->]</NOBR
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-><TR
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-ALIGN="left"
-VALIGN="TOP"
-><NOBR
-><P>pCAS2JF</NOBR
-></TD
-><TD
-ALIGN="left"
-VALIGN="TOP"
-><P>1.2 kb EcoRI/BamHI PCR fragment <I
->hpd</I
-> in pJF119EH</TD
-><TD
-ALIGN="left"
-VALIGN="TOP"
-><P>this study</TD
-></TR
-><TR
-><TD
-ALIGN="left"
-VALIGN="TOP"
-><NOBR
-><P>pCAS8</NOBR
-></TD
-><TD
-ALIGN="left"
-VALIGN="TOP"
-><P>1.1 kb NdeI/BamHI PCR fragment <I
->ggh</I
-> in pJF119<IMG
-SRC="/giflibrary/12/Delta.gif"
-BORDER="0">N</TD
-><TD
-ALIGN="left"
-VALIGN="TOP"
-><P>this study</TD
-></TR
-><TR
-><TD
-ALIGN="left"
-VALIGN="TOP"
-><NOBR
-><P>pCAS11</NOBR
-></TD
-><TD
-ALIGN="left"
-VALIGN="TOP"
-><P>0.9 kb BglII/BamHI PCR fragment <I
->crtE</I
-> in pCAS8</TD
-><TD
-ALIGN="left"
-VALIGN="TOP"
-><P>this study</TD
-></TR
-><TR
-><TD
-ALIGN="left"
-VALIGN="TOP"
-><NOBR
-><P>pCAS12</NOBR
-></TD
-><TD
-ALIGN="left"
-VALIGN="TOP"
-><P>1.0 kb BglII/BamHI PCR fragment <I
->hpt</I
-> in pCAS11</TD
-><TD
-ALIGN="left"
-VALIGN="TOP"
-><P>this study</TD
-></TR
-><TR
-><TD
-ALIGN="left"
-VALIGN="TOP"
-><NOBR
-><P>pCAS15</NOBR
-></TD
-><TD
-ALIGN="left"
-VALIGN="TOP"
-><P>1.2 kb NdeI/BamHI PCR fragment <I
->hpd</I
-> in pAW229</TD
-><TD
-ALIGN="left"
-VALIGN="TOP"
-><P>this study</TD
-></TR
-><TR
-><TD
-ALIGN="left"
-VALIGN="TOP"
-><NOBR
-><P>pCAS18</NOBR
-></TD
-><TD
-ALIGN="left"
-VALIGN="TOP"
-><P>1.2 kb BglII/BamHI PCR fragment <I
->hpd</I
-> in pCAS11</TD
-><TD
-ALIGN="left"
-VALIGN="TOP"
-><P>this study</TD
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-><NOBR
-><P>pCAS19</NOBR
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-><TD
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-><P>1.0 kb NdeI/BamHI PCR fragment <I
->hpt</I
-> in pAW229</TD
-><TD
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-VALIGN="TOP"
-><P>this study</TD
-></TR
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-><TD
-ALIGN="left"
-VALIGN="TOP"
-><NOBR
-><P>pCAS23</NOBR
-></TD
-><TD
-ALIGN="left"
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-><P>1.0 kb NdeI/BamHI PCR fragment <I
->hpt</I
-> in pJF119<IMG
-SRC="/giflibrary/12/Delta.gif"
-BORDER="0">N</TD
-><TD
-ALIGN="left"
-VALIGN="TOP"
-><P>this study</TD
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-ALIGN="left"
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-><NOBR
-><P>pCAS24</NOBR
-></TD
-><TD
-ALIGN="left"
-VALIGN="TOP"
-><P>0.9 kb NdeI/BamHI PCR fragment <I
->crtE</I
-> in pCAS23</TD
-><TD
-ALIGN="left"
-VALIGN="TOP"
-><P>this study</TD
-></TR
-><TR
-><TD
-ALIGN="left"
-VALIGN="TOP"
-><NOBR
-><P>pCAS27</NOBR
-></TD
-><TD
-ALIGN="left"
-VALIGN="TOP"
-><P>1.1 kb NdeI/BamHI PCR fragment <I
->cyc-sy</I
-> in pAW229</TD
-><TD
-ALIGN="left"
-VALIGN="TOP"
-><P>this study</TD
-></TR
-><TR
-><TD
-ALIGN="left"
-VALIGN="TOP"
-><NOBR
-><P>pCAS29</NOBR
-></TD
-><TD
-ALIGN="left"
-VALIGN="TOP"
-><P>1.2 kb BglII/BamHI PCR fragment <I
->hpd</I
-> in pCAS18</TD
-><TD
-ALIGN="left"
-VALIGN="TOP"
-><P>this study</TD
-></TR
-><TR
-><TD
-ALIGN="left"
-VALIGN="TOP"
-><NOBR
-><P>pCAS30</NOBR
-></TD
-><TD
-ALIGN="left"
-VALIGN="TOP"
-><P>0.9 kb NdeI/BamHI PCR fragment <I
->crtE</I
-> in pJF119<IMG
-SRC="/giflibrary/12/Delta.gif"
-BORDER="0">N</TD
-><TD
-ALIGN="left"
-VALIGN="TOP"
-><P>this study</TD
-></TR
-><TR
-><TD
-ALIGN="left"
-VALIGN="TOP"
-><NOBR
-><P>pCAS37</NOBR
-></TD
-><TD
-ALIGN="left"
-VALIGN="TOP"
-><P>1.2 kb BglII/BamHI PCR fragment <I
->hpd</I
-> in pCAS24</TD
-><TD
-ALIGN="left"
-VALIGN="TOP"
-><P>this study</TD
-></TR
-><TR
-><TD
-ALIGN="left"
-VALIGN="TOP"
-><NOBR
-><P>pCAS47</NOBR
-></TD
-><TD
-ALIGN="left"
-VALIGN="TOP"
-><P>1.3 kb BglII PCR fragment <I
->vte1</I
-> in pCAS29</TD
-><TD
-ALIGN="left"
-VALIGN="TOP"
-><P>this study</TD
-></TR
-></TABLE
-></TD
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->&nbsp;&nbsp;&nbsp;[a] Commercial strain. [b] Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ). [c] A kind gift from Prof. Forchhammer, Universit&auml;t-Gie&szlig;en (Germany).<BR></FONT
-></TD
-></TR
-></TABLE
-></html>