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<p>Relevant properties or genotype</TH>
<p>Relevant properties or genotype</TH>
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<th ALIGN="left" VALIGN="TOP"><NOBR>
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<th ALIGN="left" VALIGN="TOP">
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<p>Source</NOBR></TH></TR>
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<p>Source</TH></TR>
<tr>
<tr>
<td COLSPAN="3">
<td COLSPAN="3">
<hr size="1"></TD></TR>
<hr size="1"></TD></TR>
<tr>
<tr>
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<td ALIGN="left" VALIGN="TOP"><NOBR>
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<td ALIGN="left" VALIGN="TOP">
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<p>Strains</NOBR></TD>
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<p>Strains</TD>
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<td ALIGN="left" VALIGN="TOP"><NOBR></NOBR></TD>
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<td ALIGN="left" VALIGN="TOP"></TD>
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<td ALIGN="left" VALIGN="TOP"><NOBR></NOBR></TD></TR>
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<p>F<SUP>-</SUP>, <I><IMG SRC="/giflibrary/12/phgr.gif" BORDER="0" ALIGN="ABSBOTTOM"></I>80d, <I>lacZ</I><IMG SRC="/giflibrary/12/Delta.gif" BORDER="0">M15, <I>endA</I>1, <I>recA</I>1, <I>hsdR</I>17(r<SUB>K</SUB><SUP>-</SUP>m<SUB>K</SUB><SUP>-</SUP>), <I>supE</I>44, <I>thi</I>-1, <I>gyrA</I>96, <I>relA</I>1, <IMG SRC="/giflibrary/12/Delta.gif" BORDER="0">(<I>lacZYA-argF</I>)U169</TD>
<p>F<SUP>-</SUP>, <I><IMG SRC="/giflibrary/12/phgr.gif" BORDER="0" ALIGN="ABSBOTTOM"></I>80d, <I>lacZ</I><IMG SRC="/giflibrary/12/Delta.gif" BORDER="0">M15, <I>endA</I>1, <I>recA</I>1, <I>hsdR</I>17(r<SUB>K</SUB><SUP>-</SUP>m<SUB>K</SUB><SUP>-</SUP>), <I>supE</I>44, <I>thi</I>-1, <I>gyrA</I>96, <I>relA</I>1, <IMG SRC="/giflibrary/12/Delta.gif" BORDER="0">(<I>lacZYA-argF</I>)U169</TD>
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<td ALIGN="left" VALIGN="TOP"><NOBR>
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<p>[a]</NOBR></TD></TR>
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<p>[a]</TD></TR>
<tr>
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<p>F<SUP>-</SUP>, <I>lacZ<IMG SRC="/giflibrary/12/Delta.gif" BORDER="0">M15, thi</I>-1, <I>lac<SUP>-</SUP></I>, <I>mtl<SUP>-</SUP></I>, <I>recA<SUP>+</SUP></I> , Km<SUP>R</SUP></TD>
<p>F<SUP>-</SUP>, <I>lacZ<IMG SRC="/giflibrary/12/Delta.gif" BORDER="0">M15, thi</I>-1, <I>lac<SUP>-</SUP></I>, <I>mtl<SUP>-</SUP></I>, <I>recA<SUP>+</SUP></I> , Km<SUP>R</SUP></TD>
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<td ALIGN="left" VALIGN="TOP"><NOBR>
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<td ALIGN="left" VALIGN="TOP">
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<p>[a]</NOBR></TD></TR>
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<p>[a]</TD></TR>
<tr>
<tr>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
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<p>type strain</TD>
<p>type strain</TD>
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<td ALIGN="left" VALIGN="TOP"><NOBR>
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<td ALIGN="left" VALIGN="TOP">
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<p>[b]</NOBR></TD></TR>
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<p>[b]</TD></TR>
<tr>
<tr>
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<td ALIGN="left" VALIGN="TOP">
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<p>type strain</TD>
<p>type strain</TD>
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<td ALIGN="left" VALIGN="TOP"><NOBR>
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<td ALIGN="left" VALIGN="TOP">
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<p>[c]</NOBR></TD></TR>
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<p>[c]</TD></TR>
<tr>
<tr>
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<td ALIGN="left" VALIGN="TOP"><NOBR>
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<td ALIGN="left" VALIGN="TOP">
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<p>Plasmids</NOBR></TD>
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<p>Plasmids</TD>
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<td ALIGN="left" VALIGN="TOP"><NOBR></NOBR></TD>
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<td ALIGN="left" VALIGN="TOP"></TD>
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<td ALIGN="left" VALIGN="TOP"><NOBR></NOBR></TD></TR>
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<tr>
<tr>
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<td ALIGN="left" VALIGN="TOP"><NOBR>
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<p>pCAR16</NOBR></TD>
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<p>pCAR16</TD>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
<p><IMG SRC="/giflibrary/12/beta.gif" BORDER="0" ALIGN="ABSBOTTOM">-carotene biosynthesis gene cluster from <I>Pantoea ananatis</I> in pUC19, Amp<SUP>R</SUP></TD>
<p><IMG SRC="/giflibrary/12/beta.gif" BORDER="0" ALIGN="ABSBOTTOM">-carotene biosynthesis gene cluster from <I>Pantoea ananatis</I> in pUC19, Amp<SUP>R</SUP></TD>
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<td ALIGN="left" VALIGN="TOP"><NOBR>
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<td ALIGN="left" VALIGN="TOP">
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<p>[<A HREF="#BIB27">27</A>]</NOBR></TD></TR>
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<p>[<A HREF="#BIB27">27</A>]</TD></TR>
<tr>
<tr>
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<td ALIGN="left" VALIGN="TOP"><NOBR>
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<td ALIGN="left" VALIGN="TOP">
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<p>pJF119EH</NOBR></TD>
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<p>pJF119EH</TD>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
<p>cloning vector, tac-promoter, IPTG inducible, Amp<SUP>R</SUP></TD>
<p>cloning vector, tac-promoter, IPTG inducible, Amp<SUP>R</SUP></TD>
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<td ALIGN="left" VALIGN="TOP"><NOBR>
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<td ALIGN="left" VALIGN="TOP">
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<p>[<A HREF="#BIB25">25</A>]</NOBR></TD></TR>
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<p>[<A HREF="#BIB25">25</A>]</TD></TR>
<tr>
<tr>
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<td ALIGN="left" VALIGN="TOP"><NOBR>
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<td ALIGN="left" VALIGN="TOP">
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<p>pJF119<IMG SRC="/giflibrary/12/Delta.gif" BORDER="0">N</NOBR></TD>
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<p>pJF119<IMG SRC="/giflibrary/12/Delta.gif" BORDER="0">N</TD>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
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<p>this study</TD></TR>
<p>this study</TD></TR>
<tr>
<tr>
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<td ALIGN="left" VALIGN="TOP"><NOBR>
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<td ALIGN="left" VALIGN="TOP">
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<p>pAW229</NOBR></TD>
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<p>pAW229</TD>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
<p>cloning vector, RBS, <I>rha</I>-promoter, <FONT SIZE="-1">L</FONT>-rhamnose inducible, Cm<SUP>R</SUP></TD>
<p>cloning vector, RBS, <I>rha</I>-promoter, <FONT SIZE="-1">L</FONT>-rhamnose inducible, Cm<SUP>R</SUP></TD>
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<td ALIGN="left" VALIGN="TOP"><NOBR>
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<td ALIGN="left" VALIGN="TOP">
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<p>[<A HREF="#BIB26">26</A>]</NOBR></TD></TR>
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<p>[<A HREF="#BIB26">26</A>]</TD></TR>
<tr>
<tr>
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<td ALIGN="left" VALIGN="TOP"><NOBR>
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<td ALIGN="left" VALIGN="TOP">
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<p>pQE31-VTE1</NOBR></TD>
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<p>pQE31-VTE1</TD>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
<p>tocopherol-cyclase (vte1) from <I>Arabidopsis thaliana</I> in pQE31, IPTG inducible, Amp<SUP>R</SUP></TD>
<p>tocopherol-cyclase (vte1) from <I>Arabidopsis thaliana</I> in pQE31, IPTG inducible, Amp<SUP>R</SUP></TD>
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<td ALIGN="left" VALIGN="TOP"><NOBR>
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<td ALIGN="left" VALIGN="TOP">
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<p>[<A HREF="#BIB18A">18a</A>]</NOBR></TD></TR>
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<p>[<A HREF="#BIB18A">18a</A>]</TD></TR>
<tr>
<tr>
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<td ALIGN="left" VALIGN="TOP"><NOBR>
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<td ALIGN="left" VALIGN="TOP">
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<p>pCAS2JF</NOBR></TD>
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<p>pCAS2JF</TD>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
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<p>this study</TD></TR>
<p>this study</TD></TR>
<tr>
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<td ALIGN="left" VALIGN="TOP"><NOBR>
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<td ALIGN="left" VALIGN="TOP">
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<p>pCAS8</NOBR></TD>
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<p>pCAS8</TD>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
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<p>this study</TD></TR>
<p>this study</TD></TR>
<tr>
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<td ALIGN="left" VALIGN="TOP">
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<p>pCAS11</NOBR></TD>
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<p>pCAS11</TD>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
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<p>this study</TD></TR>
<p>this study</TD></TR>
<tr>
<tr>
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<td ALIGN="left" VALIGN="TOP"><NOBR>
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<td ALIGN="left" VALIGN="TOP">
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<p>pCAS12</NOBR></TD>
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<p>pCAS12</TD>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
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<p>this study</TD></TR>
<p>this study</TD></TR>
<tr>
<tr>
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<p>pCAS15</NOBR></TD>
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<p>pCAS15</TD>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
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<p>this study</TD></TR>
<p>this study</TD></TR>
<tr>
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<p>pCAS18</NOBR></TD>
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<p>pCAS18</TD>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
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<p>this study</TD></TR>
<p>this study</TD></TR>
<tr>
<tr>
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<td ALIGN="left" VALIGN="TOP"><NOBR>
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<td ALIGN="left" VALIGN="TOP">
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<p>pCAS19</NOBR></TD>
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<p>pCAS19</TD>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
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<p>this study</TD></TR>
<p>this study</TD></TR>
<tr>
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<p>pCAS23</NOBR></TD>
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<p>pCAS23</TD>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
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<p>this study</TD></TR>
<p>this study</TD></TR>
<tr>
<tr>
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<td ALIGN="left" VALIGN="TOP"><NOBR>
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<td ALIGN="left" VALIGN="TOP">
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<p>pCAS24</NOBR></TD>
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<p>pCAS24</TD>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
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<p>this study</TD></TR>
<p>this study</TD></TR>
<tr>
<tr>
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<td ALIGN="left" VALIGN="TOP"><NOBR>
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<td ALIGN="left" VALIGN="TOP">
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<p>pCAS27</NOBR></TD>
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<p>pCAS27</TD>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
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<p>this study</TD></TR>
<p>this study</TD></TR>
<tr>
<tr>
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<td ALIGN="left" VALIGN="TOP"><NOBR>
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<td ALIGN="left" VALIGN="TOP">
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<p>pCAS29</NOBR></TD>
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<p>pCAS29</TD>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
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<p>this study</TD></TR>
<p>this study</TD></TR>
<tr>
<tr>
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<td ALIGN="left" VALIGN="TOP"><NOBR>
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<p>pCAS30</NOBR></TD>
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<p>pCAS30</TD>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
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<p>this study</TD></TR>
<p>this study</TD></TR>
<tr>
<tr>
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<p>pCAS37</NOBR></TD>
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<p>pCAS37</TD>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
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<p>this study</TD></TR>
<p>this study</TD></TR>
<tr>
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<td ALIGN="left" VALIGN="TOP"><NOBR>
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<p>pCAS47</NOBR></TD>
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<p>pCAS47</TD>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">

Revision as of 21:26, 19 July 2009

 
Table 1. Strains and plasmids used in this work.

Strain or plasmid

Relevant properties or genotype

Source


Strains

E. coli DH5

F-, 80d, lacZM15, endA1, recA1, hsdR17(rK-mK-), supE44, thi-1, gyrA96, relA1, (lacZYA-argF)U169

[a]

E. coli M15 [pREP4]

F-, lacZM15, thi-1, lac-, mtl-, recA+ , KmR

[a]

Pseudomonas putida KT2440

type strain

[b]

Synechocystis sp. PCC 6803

type strain

[c]

Plasmids

pCAR16

-carotene biosynthesis gene cluster from Pantoea ananatis in pUC19, AmpR

[27]

pJF119EH

cloning vector, tac-promoter, IPTG inducible, AmpR

[25]

pJF119N

cloning vector, RBS, tac-promoter, IPTG inducible, AmpR

this study

pAW229

cloning vector, RBS, rha-promoter, L-rhamnose inducible, CmR

[26]

pQE31-VTE1

tocopherol-cyclase (vte1) from Arabidopsis thaliana in pQE31, IPTG inducible, AmpR

[18a]

pCAS2JF

1.2 kb EcoRI/BamHI PCR fragment hpd in pJF119EH

this study

pCAS8

1.1 kb NdeI/BamHI PCR fragment ggh in pJF119N

this study

pCAS11

0.9 kb BglII/BamHI PCR fragment crtE in pCAS8

this study

pCAS12

1.0 kb BglII/BamHI PCR fragment hpt in pCAS11

this study

pCAS15

1.2 kb NdeI/BamHI PCR fragment hpd in pAW229

this study

pCAS18

1.2 kb BglII/BamHI PCR fragment hpd in pCAS11

this study

pCAS19

1.0 kb NdeI/BamHI PCR fragment hpt in pAW229

this study

pCAS23

1.0 kb NdeI/BamHI PCR fragment hpt in pJF119N

this study

pCAS24

0.9 kb NdeI/BamHI PCR fragment crtE in pCAS23

this study

pCAS27

1.1 kb NdeI/BamHI PCR fragment cyc-sy in pAW229

this study

pCAS29

1.2 kb BglII/BamHI PCR fragment hpd in pCAS18

this study

pCAS30

0.9 kb NdeI/BamHI PCR fragment crtE in pJF119N

this study

pCAS37

1.2 kb BglII/BamHI PCR fragment hpd in pCAS24

this study

pCAS47

1.3 kb BglII PCR fragment vte1 in pCAS29

this study


   [a] Commercial strain. [b] Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ). [c] A kind gift from Prof. Forchhammer, Universität-Gießen (Germany).

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