Template:HugeFriggingTable

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<p>Strain or plasmid</TH>
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Strain or plasmid</TH>
<th ALIGN="left" VALIGN="TOP">
<th ALIGN="left" VALIGN="TOP">
-
<p>Relevant properties or genotype</TH>
+
Relevant properties or genotype</TH>
<th ALIGN="left" VALIGN="TOP">
<th ALIGN="left" VALIGN="TOP">
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<p>Source</TH></TR>
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Source</TH></TR>
<tr>
<tr>
<td COLSPAN="3">
<td COLSPAN="3">
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<p>Strains</TD>
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Strains</TD>
<td ALIGN="left" VALIGN="TOP"></TD>
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<td ALIGN="left" VALIGN="TOP"></TD></TR>
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<td ALIGN="left" VALIGN="TOP">
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<p><I>E. coli</I> DH5<IMG SRC="/giflibrary/12/alpha.gif" BORDER="0"></TD>
+
<I>E. coli</I> DH5<IMG SRC="/giflibrary/12/alpha.gif" BORDER="0"></TD>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
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<p>F<SUP>-</SUP>, <I><IMG SRC="/giflibrary/12/phgr.gif" BORDER="0" ALIGN="ABSBOTTOM"></I>80d, <I>lacZ</I><IMG SRC="/giflibrary/12/Delta.gif" BORDER="0">M15, <I>endA</I>1, <I>recA</I>1, <I>hsdR</I>17(r<SUB>K</SUB><SUP>-</SUP>m<SUB>K</SUB><SUP>-</SUP>), <I>supE</I>44, <I>thi</I>-1, <I>gyrA</I>96, <I>relA</I>1, <IMG SRC="/giflibrary/12/Delta.gif" BORDER="0">(<I>lacZYA-argF</I>)U169</TD>
+
F<SUP>-</SUP>, <I><IMG SRC="/giflibrary/12/phgr.gif" BORDER="0" ALIGN="ABSBOTTOM"></I>80d, <I>lacZ</I><IMG SRC="/giflibrary/12/Delta.gif" BORDER="0">M15, <I>endA</I>1, <I>recA</I>1, <I>hsdR</I>17(r<SUB>K</SUB><SUP>-</SUP>m<SUB>K</SUB><SUP>-</SUP>), <I>supE</I>44, <I>thi</I>-1, <I>gyrA</I>96, <I>relA</I>1, <IMG SRC="/giflibrary/12/Delta.gif" BORDER="0">(<I>lacZYA-argF</I>)U169</TD>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
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<p>[a]</TD></TR>
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[a]</TD></TR>
<tr>
<tr>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
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<p><I>E. coli</I> M15 [pREP4]</TD>
+
<I>E. coli</I> M15 [pREP4]</TD>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
-
<p>F<SUP>-</SUP>, <I>lacZ<IMG SRC="/giflibrary/12/Delta.gif" BORDER="0">M15, thi</I>-1, <I>lac<SUP>-</SUP></I>, <I>mtl<SUP>-</SUP></I>, <I>recA<SUP>+</SUP></I> , Km<SUP>R</SUP></TD>
+
F<SUP>-</SUP>, <I>lacZ<IMG SRC="/giflibrary/12/Delta.gif" BORDER="0">M15, thi</I>-1, <I>lac<SUP>-</SUP></I>, <I>mtl<SUP>-</SUP></I>, <I>recA<SUP>+</SUP></I> , Km<SUP>R</SUP></TD>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
-
<p>[a]</TD></TR>
+
[a]</TD></TR>
<tr>
<tr>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
-
<p><I>Pseudomonas putida</I> KT2440</TD>
+
<I>Pseudomonas putida</I> KT2440</TD>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
-
<p>type strain</TD>
+
type strain</TD>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
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<p>[b]</TD></TR>
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[b]</TD></TR>
<tr>
<tr>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
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<p><I>Synechocystis</I> sp. PCC 6803</TD>
+
<I>Synechocystis</I> sp. PCC 6803</TD>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
-
<p>type strain</TD>
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type strain</TD>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
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<p>[c]</TD></TR>
+
[c]</TD></TR>
<tr>
<tr>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
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<p>Plasmids</TD>
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Plasmids</TD>
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<td ALIGN="left" VALIGN="TOP"></TD>
<td ALIGN="left" VALIGN="TOP"></TD></TR>
<td ALIGN="left" VALIGN="TOP"></TD></TR>
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<p>pCAR16</TD>
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pCAR16</TD>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
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<p><IMG SRC="/giflibrary/12/beta.gif" BORDER="0" ALIGN="ABSBOTTOM">-carotene biosynthesis gene cluster from <I>Pantoea ananatis</I> in pUC19, Amp<SUP>R</SUP></TD>
+
<IMG SRC="/giflibrary/12/beta.gif" BORDER="0" ALIGN="ABSBOTTOM">-carotene biosynthesis gene cluster from <I>Pantoea ananatis</I> in pUC19, Amp<SUP>R</SUP></TD>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
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<p>[<A HREF="#BIB27">27</A>]</TD></TR>
+
[<A HREF="#BIB27">27</A>]</TD></TR>
<tr>
<tr>
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<td ALIGN="left" VALIGN="TOP">
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<p>pJF119EH</TD>
+
pJF119EH</TD>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
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<p>cloning vector, tac-promoter, IPTG inducible, Amp<SUP>R</SUP></TD>
+
cloning vector, tac-promoter, IPTG inducible, Amp<SUP>R</SUP></TD>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
-
<p>[<A HREF="#BIB25">25</A>]</TD></TR>
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[<A HREF="#BIB25">25</A>]</TD></TR>
<tr>
<tr>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
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<p>pJF119<IMG SRC="/giflibrary/12/Delta.gif" BORDER="0">N</TD>
+
pJF119<IMG SRC="/giflibrary/12/Delta.gif" BORDER="0">N</TD>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
-
<p>cloning vector, RBS, tac-promoter, IPTG inducible, Amp<SUP>R</SUP></TD>
+
cloning vector, RBS, tac-promoter, IPTG inducible, Amp<SUP>R</SUP></TD>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
-
<p>this study</TD></TR>
+
this study</TD></TR>
<tr>
<tr>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
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<p>pAW229</TD>
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pAW229</TD>
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<td ALIGN="left" VALIGN="TOP">
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<p>cloning vector, RBS, <I>rha</I>-promoter, <FONT SIZE="-1">L</FONT>-rhamnose inducible, Cm<SUP>R</SUP></TD>
+
cloning vector, RBS, <I>rha</I>-promoter, <FONT SIZE="-1">L</FONT>-rhamnose inducible, Cm<SUP>R</SUP></TD>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
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<p>[<A HREF="#BIB26">26</A>]</TD></TR>
+
[<A HREF="#BIB26">26</A>]</TD></TR>
<tr>
<tr>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
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<p>pQE31-VTE1</TD>
+
pQE31-VTE1</TD>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
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<p>tocopherol-cyclase (vte1) from <I>Arabidopsis thaliana</I> in pQE31, IPTG inducible, Amp<SUP>R</SUP></TD>
+
tocopherol-cyclase (vte1) from <I>Arabidopsis thaliana</I> in pQE31, IPTG inducible, Amp<SUP>R</SUP></TD>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
-
<p>[<A HREF="#BIB18A">18a</A>]</TD></TR>
+
[<A HREF="#BIB18A">18a</A>]</TD></TR>
<tr>
<tr>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
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<p>pCAS2JF</TD>
+
pCAS2JF</TD>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
-
<p>1.2 kb EcoRI/BamHI PCR fragment <I>hpd</I> in pJF119EH</TD>
+
1.2 kb EcoRI/BamHI PCR fragment <I>hpd</I> in pJF119EH</TD>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
-
<p>this study</TD></TR>
+
this study</TD></TR>
<tr>
<tr>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
-
<p>pCAS8</TD>
+
pCAS8</TD>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
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<p>1.1 kb NdeI/BamHI PCR fragment <I>ggh</I> in pJF119<IMG SRC="/giflibrary/12/Delta.gif" BORDER="0">N</TD>
+
1.1 kb NdeI/BamHI PCR fragment <I>ggh</I> in pJF119<IMG SRC="/giflibrary/12/Delta.gif" BORDER="0">N</TD>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
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<p>this study</TD></TR>
+
this study</TD></TR>
<tr>
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<p>pCAS11</TD>
+
pCAS11</TD>
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<td ALIGN="left" VALIGN="TOP">
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<p>0.9 kb BglII/BamHI PCR fragment <I>crtE</I> in pCAS8</TD>
+
0.9 kb BglII/BamHI PCR fragment <I>crtE</I> in pCAS8</TD>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
-
<p>this study</TD></TR>
+
this study</TD></TR>
<tr>
<tr>
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<td ALIGN="left" VALIGN="TOP">
-
<p>pCAS12</TD>
+
pCAS12</TD>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
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<p>1.0 kb BglII/BamHI PCR fragment <I>hpt</I> in pCAS11</TD>
+
1.0 kb BglII/BamHI PCR fragment <I>hpt</I> in pCAS11</TD>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
-
<p>this study</TD></TR>
+
this study</TD></TR>
<tr>
<tr>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
-
<p>pCAS15</TD>
+
pCAS15</TD>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
-
<p>1.2 kb NdeI/BamHI PCR fragment <I>hpd</I> in pAW229</TD>
+
1.2 kb NdeI/BamHI PCR fragment <I>hpd</I> in pAW229</TD>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
-
<p>this study</TD></TR>
+
this study</TD></TR>
<tr>
<tr>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
-
<p>pCAS18</TD>
+
pCAS18</TD>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
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<p>1.2 kb BglII/BamHI PCR fragment <I>hpd</I> in pCAS11</TD>
+
1.2 kb BglII/BamHI PCR fragment <I>hpd</I> in pCAS11</TD>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
-
<p>this study</TD></TR>
+
this study</TD></TR>
<tr>
<tr>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
-
<p>pCAS19</TD>
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pCAS19</TD>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
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<p>1.0 kb NdeI/BamHI PCR fragment <I>hpt</I> in pAW229</TD>
+
1.0 kb NdeI/BamHI PCR fragment <I>hpt</I> in pAW229</TD>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
-
<p>this study</TD></TR>
+
this study</TD></TR>
<tr>
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<p>pCAS23</TD>
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pCAS23</TD>
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<td ALIGN="left" VALIGN="TOP">
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<p>1.0 kb NdeI/BamHI PCR fragment <I>hpt</I> in pJF119<IMG SRC="/giflibrary/12/Delta.gif" BORDER="0">N</TD>
+
1.0 kb NdeI/BamHI PCR fragment <I>hpt</I> in pJF119<IMG SRC="/giflibrary/12/Delta.gif" BORDER="0">N</TD>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
-
<p>this study</TD></TR>
+
this study</TD></TR>
<tr>
<tr>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
-
<p>pCAS24</TD>
+
pCAS24</TD>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
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<p>0.9 kb NdeI/BamHI PCR fragment <I>crtE</I> in pCAS23</TD>
+
0.9 kb NdeI/BamHI PCR fragment <I>crtE</I> in pCAS23</TD>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
-
<p>this study</TD></TR>
+
this study</TD></TR>
<tr>
<tr>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
-
<p>pCAS27</TD>
+
pCAS27</TD>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
-
<p>1.1 kb NdeI/BamHI PCR fragment <I>cyc-sy</I> in pAW229</TD>
+
1.1 kb NdeI/BamHI PCR fragment <I>cyc-sy</I> in pAW229</TD>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
-
<p>this study</TD></TR>
+
this study</TD></TR>
<tr>
<tr>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
-
<p>pCAS29</TD>
+
pCAS29</TD>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
-
<p>1.2 kb BglII/BamHI PCR fragment <I>hpd</I> in pCAS18</TD>
+
1.2 kb BglII/BamHI PCR fragment <I>hpd</I> in pCAS18</TD>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
-
<p>this study</TD></TR>
+
this study</TD></TR>
<tr>
<tr>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
-
<p>pCAS30</TD>
+
pCAS30</TD>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
-
<p>0.9 kb NdeI/BamHI PCR fragment <I>crtE</I> in pJF119<IMG SRC="/giflibrary/12/Delta.gif" BORDER="0">N</TD>
+
0.9 kb NdeI/BamHI PCR fragment <I>crtE</I> in pJF119<IMG SRC="/giflibrary/12/Delta.gif" BORDER="0">N</TD>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
-
<p>this study</TD></TR>
+
this study</TD></TR>
<tr>
<tr>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
-
<p>pCAS37</TD>
+
pCAS37</TD>
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<td ALIGN="left" VALIGN="TOP">
-
<p>1.2 kb BglII/BamHI PCR fragment <I>hpd</I> in pCAS24</TD>
+
1.2 kb BglII/BamHI PCR fragment <I>hpd</I> in pCAS24</TD>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
-
<p>this study</TD></TR>
+
this study</TD></TR>
<tr>
<tr>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
-
<p>pCAS47</TD>
+
pCAS47</TD>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
-
<p>1.3 kb BglII PCR fragment <I>vte1</I> in pCAS29</TD>
+
1.3 kb BglII PCR fragment <I>vte1</I> in pCAS29</TD>
<td ALIGN="left" VALIGN="TOP">
<td ALIGN="left" VALIGN="TOP">
-
<p>this study</TD></TR></TABLE></TD></TR>
+
this study</TD></TR></TABLE></TD></TR>
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<td>

Revision as of 20:27, 19 July 2009

 
Table 1. Strains and plasmids used in this work.

Strain or plasmid Relevant properties or genotype Source

Strains
E. coli DH5 F-, 80d, lacZM15, endA1, recA1, hsdR17(rK-mK-), supE44, thi-1, gyrA96, relA1, (lacZYA-argF)U169 [a]
E. coli M15 [pREP4] F-, lacZM15, thi-1, lac-, mtl-, recA+ , KmR [a]
Pseudomonas putida KT2440 type strain [b]
Synechocystis sp. PCC 6803 type strain [c]
Plasmids
pCAR16 -carotene biosynthesis gene cluster from Pantoea ananatis in pUC19, AmpR [27]
pJF119EH cloning vector, tac-promoter, IPTG inducible, AmpR [25]
pJF119N cloning vector, RBS, tac-promoter, IPTG inducible, AmpR this study
pAW229 cloning vector, RBS, rha-promoter, L-rhamnose inducible, CmR [26]
pQE31-VTE1 tocopherol-cyclase (vte1) from Arabidopsis thaliana in pQE31, IPTG inducible, AmpR [18a]
pCAS2JF 1.2 kb EcoRI/BamHI PCR fragment hpd in pJF119EH this study
pCAS8 1.1 kb NdeI/BamHI PCR fragment ggh in pJF119N this study
pCAS11 0.9 kb BglII/BamHI PCR fragment crtE in pCAS8 this study
pCAS12 1.0 kb BglII/BamHI PCR fragment hpt in pCAS11 this study
pCAS15 1.2 kb NdeI/BamHI PCR fragment hpd in pAW229 this study
pCAS18 1.2 kb BglII/BamHI PCR fragment hpd in pCAS11 this study
pCAS19 1.0 kb NdeI/BamHI PCR fragment hpt in pAW229 this study
pCAS23 1.0 kb NdeI/BamHI PCR fragment hpt in pJF119N this study
pCAS24 0.9 kb NdeI/BamHI PCR fragment crtE in pCAS23 this study
pCAS27 1.1 kb NdeI/BamHI PCR fragment cyc-sy in pAW229 this study
pCAS29 1.2 kb BglII/BamHI PCR fragment hpd in pCAS18 this study
pCAS30 0.9 kb NdeI/BamHI PCR fragment crtE in pJF119N this study
pCAS37 1.2 kb BglII/BamHI PCR fragment hpd in pCAS24 this study
pCAS47 1.3 kb BglII PCR fragment vte1 in pCAS29 this study

   [a] Commercial strain. [b] Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ). [c] A kind gift from Prof. Forchhammer, Universität-Gießen (Germany).

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