Template:Imperial/Protocols: Difference between revisions

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m (New page: {| cellpadding="1" style="background:#66aadd; border:1px solid #66aadd; color:black" align="left" width=50% |width="100%"| <font size=4px><font color=white>'''1. Transformation Protocols f...)
 
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{| cellpadding="1" style="background:#66aadd; border:1px solid #66aadd; color:black" align="left" width=50%
<html><table cellpadding="1" style="background:#66aadd; border:1px solid #66aadd; color:black" align="left" width=50%>
|width="100%"| <font size=4px><font color=white>'''1. Transformation Protocols for integration vectors in ''Bacillus subtilis'''''
<tr><td width="100%"> <font size=4px><font color=white>'''1. Transformation Protocols for integration vectors in ''Bacillus subtilis'''''</td></tr>
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</html>*[[/Transformation_protocol_2 | Protocol 1]]<html>
*[[/Transformation_protocol_2 | Protocol 1]]
</html>*[[/Transformation_protocol_4 | Protocol 2]]<html></td></tr>
*[[/Transformation_protocol_4 | Protocol 2]]
<tr><td style="background:#66aadd"><font size=4px><font color=white>'''2. Transformation Protocol for linear DNA in ''Bacillus subtilis'''''</td></tr>
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|style="background:#66aadd"| <font size=4px><font color=white>'''2. Transformation Protocol for linear DNA in ''Bacillus subtilis'''''
</html>*Protocol<html></td></tr>
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<tr><td style="background:#66aadd"> <font size=4px><font color=white>'''3. Transformation Protocol ''E.coli'''''</td></tr>
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*Protocol
</html>*[[/XL1-Blue_preparation | Preparation of XL1-blue electrocompetent cells]]<html></td></tr>
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<tr><td style="background:#66aadd"> <font size=4px><font color=white>'''4. Motility Analysis'''</td></tr>
|style="background:#66aadd"| <font size=4px><font color=white>'''3. Transformation Protocol ''E.coli'''''
<tr><td style="background:#FFFFFF">
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</html>*[[/Protocols#Motility_Experiments | Motility Assays]]<html></td></tr>
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<tr><td style="background:#66aadd"> <font size=4px><font color=white>'''5. PCR'''</td></tr>
*[[/XL1-Blue_preparation | Preparation of XL1-blue electrocompetent cells]]
<tr><td style="background:#FFFFFF">
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</html>*[[/PCR| PCR]]<html>
|style="background:#66aadd"| <font size=4px><font color=white>'''4. Motility Analysis'''
</html>*[[/Colony_PCR| Single Colony PCR]]<html></td></tr>
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<tr><td style="background:#66aadd"> <font size=4px><font color=white>'''6. Calibration Curves'''</td></tr>
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*[[/Protocols#Motility_Experiments | Motility Assays]]
</html>*[[/Calibration-Curves| Calibration Curves]]<html>
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</html>*[[/Growth| O.D.<sub>600</sub> vs cell number]]<html>
|style="background:#66aadd"| <font size=4px><font color=white>'''5. PCR'''
</html>*[[/Fluorescence| Fluorescence vs GFPmut3b]]<html></td></tr>
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<tr><td style="background:#66aadd"> <font size=4px><font color=white>'''7. Promoter-RBS Testing Protocols'''</td></tr>
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<tr><td style="background:#FFFFFF">
*[[/PCR| PCR]]
</html>*[[/Constitutive | Constitutive Promoters and RBS]]<html>
*[[/Colony_PCR| Single Colony PCR]]
</html>*[[/Inducible | pHyperspank Promoter and RBS]]<html>
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</html>*[[/Light_Inducible | Light Inducible Promoters and RBS]]<html></td></tr>
|style="background:#66aadd"| <font size=4px><font color=white>'''6. Calibration Curves'''
<tr><td style="background:#66aadd"> <font size=4px><font color=white>'''8. Biomaterial Protocols'''</td></tr>
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</html>*Protocol<html></td></tr>
*[[/Calibration-Curves| Calibration Curves ]]
</table></html>
*[[/Growth| O.D.<sub>600</sub> vs cell number]]
*[[/Fluorescence| Fluorescence vs GFPmut3b]]
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|style="background:#66aadd"| <font size=4px><font color=white>'''7. Promoter-RBS Testing Protocols'''
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|style="background:#FFFFFF"|
*[[/Constitutive | Constitutive Promoters and RBS]]  
*[[/Inducible | pHyperspank Promoter and RBS]]
*[[/Light_Inducible | Light Inducible Promoters and RBS]]
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|style="background:#66aadd"| <font size=4px><font color=white>'''8. Biomaterial Protocols'''
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|style="background:#FFFFFF"|
*Protocol
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Revision as of 07:00, 18 September 2008

<html><table cellpadding="1" style="background:#66aadd; border:1px solid #66aadd; color:black" align="left" width=50%> <tr><td width="100%"> <font size=4px><font color=white>'''1. Transformation Protocols for integration vectors in ''Bacillus subtilis'''''</td></tr> <tr><td style="background:#FFFFFF"> </html>* Protocol 1<html> </html>* Protocol 2<html></td></tr> <tr><td style="background:#66aadd"><font size=4px><font color=white>'''2. Transformation Protocol for linear DNA in ''Bacillus subtilis'''''</td></tr> <tr><td style="background:#FFFFFF"> </html>*Protocol<html></td></tr> <tr><td style="background:#66aadd"> <font size=4px><font color=white>'''3. Transformation Protocol ''E.coli'''''</td></tr> <tr><td style="background:#FFFFFF"> </html>* Preparation of XL1-blue electrocompetent cells<html></td></tr> <tr><td style="background:#66aadd"> <font size=4px><font color=white>'''4. Motility Analysis'''</td></tr> <tr><td style="background:#FFFFFF"> </html>* Motility Assays<html></td></tr> <tr><td style="background:#66aadd"> <font size=4px><font color=white>'''5. PCR'''</td></tr> <tr><td style="background:#FFFFFF"> </html>* PCR<html> </html>* Single Colony PCR<html></td></tr> <tr><td style="background:#66aadd"> <font size=4px><font color=white>'''6. Calibration Curves'''</td></tr> <tr><td style="background:#FFFFFF"> </html>* Calibration Curves<html> </html>* O.D.600 vs cell number<html> </html>* Fluorescence vs GFPmut3b<html></td></tr> <tr><td style="background:#66aadd"> <font size=4px><font color=white>'''7. Promoter-RBS Testing Protocols'''</td></tr> <tr><td style="background:#FFFFFF"> </html>* Constitutive Promoters and RBS<html> </html>* pHyperspank Promoter and RBS<html> </html>* Light Inducible Promoters and RBS<html></td></tr> <tr><td style="background:#66aadd"> <font size=4px><font color=white>'''8. Biomaterial Protocols'''</td></tr> <tr><td style="background:#FFFFFF"> </html>*Protocol<html></td></tr> </table></html>