Template:KubkeLabEmbryo: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 11: Line 11:
<br>Species: Gallus gallus domesticus
<br>Species: Gallus gallus domesticus
<br>Embryo Name: RC-006
<br>Embryo Name: RC-006
<br>Embryo stage: 20
<br>Embryo stage: 20  
::(Stage was not confirmed, should be taken as approximate)--[[User:M Fabiana Kubke|MF Kubke]] 20:31, 13 January 2011 (EST)
<br>Staging description:
<br>Staging description:
*Allantois has vesicularized and is roughly the same size as the midbrain (indicating stage 20-21)
*Allantois has vesicularized and is roughly the same size as the midbrain (indicating stage 20-21)

Revision as of 18:31, 13 January 2011

Cranial Nerve Development Experiment

Embryo details


Species: Gallus gallus domesticus
Embryo Name: RC-006
Embryo stage: 20

(Stage was not confirmed, should be taken as approximate)--MF Kubke 20:31, 13 January 2011 (EST)


Staging description:

  • Allantois has vesicularized and is roughly the same size as the midbrain (indicating stage 20-21)
  • Contour of mid-trunk region is a straight line and not curved enough to be stage 21.

Note: RC-006 Is a rather large embryo for its stage. Tissue appeared well preserved and hence it was selected for cutting.

Experiment details


Objective: Use the embryo to create a series of cross-sectional images of the hindbrain to identify structural features.
Procedure: Complete a serial sectioning using the cryostat followed by a Nissl stain.
Comments:

Results

Images

Summary