Template:KubkeLabEmbryo: Difference between revisions
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=Embryo details= | =Embryo details= | ||
<br>Species: | <br>Species: Gallus gallus domesticus | ||
<br>Embryo Name: | <br>Embryo Name: RC-006 | ||
<br>Embryo stage: | <br>Embryo stage: 20 | ||
<br>Staging description: | <br>Staging description: | ||
*Allantois has vesicularized and is roughly the same size as the midbrain (indicating stage 20-21) | |||
*Contour of mid-trunk region is a straight line and not curved enough to be stage 21. | |||
Note: RC-006 Is a rather large embryo for its stage. Tissue appeared well preserved and hence it was selected for cutting. | |||
=Experiment details= | =Experiment details= | ||
Revision as of 19:54, 16 January 2011
| Cranial Nerve Development | Experiment |
Embryo details
Species: Gallus gallus domesticus
Embryo Name: RC-006
Embryo stage: 20
Staging description:
- Allantois has vesicularized and is roughly the same size as the midbrain (indicating stage 20-21)
- Contour of mid-trunk region is a straight line and not curved enough to be stage 21.
Note: RC-006 Is a rather large embryo for its stage. Tissue appeared well preserved and hence it was selected for cutting.
Experiment details
Objective:Use the embryo to create a series of cross-sectional images of the hindbrain to identify structural features.
Procedure:Complete a serial sectioning using the cryostat followed by a Nissl stain.
Comments: The slides used to mount the sections were 'Superfrost PLUS' slides. The sections fell off the slides. The slides were kept in Xylene solution for 3 hours prior to coverslipping. The coverslipped slides have been put in a folder labeled RC-006 in the lab.
