Template:KubkeLabEmbryo: Difference between revisions

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=Embryo details=
=Embryo details=


<br>Species: Gallus gallus domesticus
<br>'''Species''':
<br>Embryo Name:RC-009
<br>'''Embryo Name''':
<br>Embryo stage: 24 {{Note|Staging not confirmed by Supervisor}}
<br>'''Embryo stage''':
<br>Staging description:
<br>'''Staging description''':
*Elbow and knee joints are distinct. This may indicate that the embryo is greater than stage 24.
<br>'''Fixation''':
*No toe demarcation visible. This may indicate that the embryo is less than stage 26.
<br>'''Cryoprotection''':
*Toe groove on developing leg not visible. This indicates that the embryo has not reached stage 25.
<br>'''Material label and storage''':
Therefore the embryo is stage 24.
<br>Cryoprotection: Not cryoprotected. Sectioned from PFA.


=Experiment details=
=Experiment details=


<br>Objective: Analyze Nissl-stained serial sections of the embryo. 
<br>'''Objective''':
<br>Procedure:  
<br>'''Procedure''':  
*Staged the embryo according to Hamburger and Hamilton staging (1951). {{Note|This is an approximation as it was not checked}}
<br>'''Comments''':
*Under a dissection microscope the head was cleaved from the tail using the dissection scissors at the level of the wing bud. {{Note|This can not be confirmed as there was no photograph taken of the embryo prior to cutting}}
*The embryo was embedded in OCT and frozen at -19°C (in the cryostat chamber), loaded onto a chuck and incubated for an hour.
*Serial sections were cut and mounted onto the gelatin-subbed slides whilst time spent cutting the section and time spent in chamber before mounting were recorded into my lab-book.
*The sections were dried and then Nissl-stained for visualization under a microscope.
<br>Comments:I did not allow myself enough time to complete the sectioning of the embryo. I stopped halfway through the hindbrain. The other half of the hindbrain and head were discarded.


=Results=
=Results=
Half of the hindbrain and head are missing from the slides as they were discarded.
=Images=
=Images=
=Summary=


No images have been taken.
=Summary=


The objective was not achieved as I did not allow enough time to complete the cutting of the embryo.


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Latest revision as of 00:45, 27 January 2011

Cranial Nerve Development Experiment

Embryo details


Species:
Embryo Name:
Embryo stage:
Staging description:
Fixation:
Cryoprotection:
Material label and storage:

Experiment details


Objective:
Procedure:
Comments:

Results

Images

Summary

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