Template:KubkeLabEmbryo

Cranial Nerve Development

Experiment

Embryo details

Species: Gallus gallus domesticus
Embryo Name: RC-006
Embryo stage: 20
Staging description:

• Allantois has vesicularized and is roughly the same size as the midbrain (indicating stage 20-21)
• Contour of mid-trunk region is a straight line and not curved enough to be stage 21.

Note: RC-006 Is a rather large embryo for its stage. Tissue appeared well preserved and hence it was selected for cutting.

Experiment details

Objective:Use the embryo to create a series of cross-sectional images of the hindbrain to identify structural features.
Procedure:Complete a serial sectioning using the cryostat followed by a Nissl stain.
Comments: Cryostat settings: 1.5 degrees knife angle, -19 degrees C chamber and object temperature of the cryostat. The slides were kept in Xylene solution for 3 hours prior to coverslipping. The slides used to mount the sections were 'Superfrost PLUS' slides.

Results

The slides used to mount the sections were 'Superfrost PLUS' slides. The sections fell off the slides. The coverslipped slides have been put in a folder labeled RC-006 in the lab.