Template:PCR reaction
From OpenWetWare
| Reaction Mix (μl) | Thermocycling | ||
|---|---|---|---|
| H2O | {{{Water}}} | {{{Denaturing Temp}}} | {{{Denaturing Time}}} |
| Buffer | {{{Buffer}}} | {{{Denaturing Cycle Temp}}} | {{{Denaturing Cycle Time}}} |
| Forward Primer | {{{PrimerF}}} | {{{Annealing Temp}}} | {{{Annealing Time}}} |
| Reverse Primer | {{{PrimerR}}} | {{{Extension Temp}}} | {{{Extension Time}}} |
| dNTPS | {{{dNTPs}}} | Cycles | {{{Cycles}}} |
| Template | {{{Template}}} | {{{Final Extension Temp}}} | {{{Final Extension Time}}} |
| Polymerase | {{{Polymerase}}} | 4oC | {{{4CTime}}} |
| {{{Notes}}} | |||
