Template:SBB-Protocols Enz3

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(New page: ===BglII Digest of EIPCR Products===)
Current revision (19:54, 23 June 2010) (view source)
(BglII Digest of EIPCR Products)
 
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===BglII Digest of EIPCR Products===
===BglII Digest of EIPCR Products===
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'''For EIPCR products, you will only digest a portion of your purified PCR product.  This procedure is almost identical to the digestion of a PCR product with EcoRI/BamHI, but you'll use BglII instead of EcoRI/BamHI.'''
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*Set up the following reaction:
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  8.5uL of eluted PCR product
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  1uL of NEB Buffer 2
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  1uL BglII
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*Incubate at 37 degrees on the thermocycler for 1hr
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*Run an agarose gel, cut out the band, and melt with 600uL ADB buffer at 55 degrees
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*Proceed with remainder of Zymo gel purification protocol

Current revision

BglII Digest of EIPCR Products

For EIPCR products, you will only digest a portion of your purified PCR product. This procedure is almost identical to the digestion of a PCR product with EcoRI/BamHI, but you'll use BglII instead of EcoRI/BamHI.

  • Set up the following reaction:
 8.5uL of eluted PCR product
 1uL of NEB Buffer 2
 1uL BglII
  • Incubate at 37 degrees on the thermocycler for 1hr
  • Run an agarose gel, cut out the band, and melt with 600uL ADB buffer at 55 degrees
  • Proceed with remainder of Zymo gel purification protocol
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