Template:SBB-Protocols Enz3

BglII Digest of EIPCR Products

For EIPCR products, you will only digest a portion of your purified PCR product. This procedure is almost identical to the digestion of a PCR product with EcoRI/BamHI, but you'll use BglII instead of EcoRI/BamHI.

• Set up the following reaction:

 8.5uL of eluted PCR product
 1uL of NEB Buffer 2
 0.5uL BglII

• Incubate at 37 degrees on the thermocycler for 1hr
• Run an agarose gel, cut out the band, and melt with 600uL ADB buffer at 55 degrees
• Proceed with remainder of Zymo gel purification protocol