BglII Digest of EIPCR Products
For EIPCR products, you will only digest a portion of your purified PCR product. This procedure is almost identical to the digestion of a PCR product with EcoRI/BamHI, but you'll use BglII instead of EcoRI/BamHI.
- Set up the following reaction:
8.5uL of eluted PCR product 1uL of NEB Buffer 2 0.5uL BglII
- Incubate at 37 degrees on the thermocycler for 1hr
- Run an agarose gel, cut out the band, and melt with 600uL ADB buffer at 55 degrees
- Proceed with remainder of Zymo gel purification protocol