Template:SBB-Protocols Enz4

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(New page: ===Ligation of EcoRI/BamHI digests===)
Current revision (00:02, 4 February 2009) (view source)
(Ligation of EcoRI/BamHI digests)
 
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===Ligation of EcoRI/BamHI digests===
===Ligation of EcoRI/BamHI digests===
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*Set up the following reaction:
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  6.5uL ddH2O
 +
  1uL T4 DNA Ligase Buffer (small red or black-striped tubes)
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  1uL Vector digest
 +
  1uL Insert digest
 +
  0.5uL T4 DNA Ligase
 +
*Pound upside down on the bench to mix
 +
*Give it a quick spin to send it back to the bottom of the tube
 +
*Incubate on the benchtop for 30min
 +
*Put on ice and proceed to the transformation

Current revision

Ligation of EcoRI/BamHI digests

  • Set up the following reaction:
 6.5uL ddH2O
 1uL T4 DNA Ligase Buffer (small red or black-striped tubes)
 1uL Vector digest
 1uL Insert digest
 0.5uL T4 DNA Ligase
  • Pound upside down on the bench to mix
  • Give it a quick spin to send it back to the bottom of the tube
  • Incubate on the benchtop for 30min
  • Put on ice and proceed to the transformation
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