Template:SBB-Protocols Enz5: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
JCAnderson (talk | contribs) (New page: ===Ligation of EIPCR digests===) |
JCAnderson (talk | contribs) |
||
| Line 1: | Line 1: | ||
===Ligation of EIPCR digests=== | ===Ligation of EIPCR digests=== | ||
*Set up the following reaction: | |||
7.5uL ddH2O | |||
1uL T4 DNA Ligase Buffer (small red or black-striped tubes) | |||
1uL PCR product digest | |||
0.5uL T4 DNA Ligase | |||
*Pound upside down on the bench to mix | |||
*Give it a quick spin to send it back to the bottom of the tube | |||
*Incubate on the benchtop for 30min | |||
*Put on ice and proceed to the transformation | |||
Latest revision as of 22:03, 3 February 2009
Ligation of EIPCR digests
- Set up the following reaction:
7.5uL ddH2O 1uL T4 DNA Ligase Buffer (small red or black-striped tubes) 1uL PCR product digest 0.5uL T4 DNA Ligase
- Pound upside down on the bench to mix
- Give it a quick spin to send it back to the bottom of the tube
- Incubate on the benchtop for 30min
- Put on ice and proceed to the transformation
