Template:SBB-Protocols Enz6

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(New page: Set up the following 10uL reaction in a PCR tube: <pre> 6.5 uL ddH2O 2uL Miniprepped plasmid 1uL 10x NEB Buffer 2 0.5uL EcoRI 0.5uL BamHI (for parts >250bp) or XhoI (for parts <250bp) </pr...)
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*Take a picture of the gel
*Take a picture of the gel
*Calculate the expected fragment sizes
*Calculate the expected fragment sizes
-
*Are the claculated sizes consistent with the bands on the gel?
+
*Are the calculated sizes consistent with the bands on the gel?

Revision as of 17:15, 2 March 2009

Set up the following 10uL reaction in a PCR tube:

6.5 uL ddH2O
2uL Miniprepped plasmid
1uL 10x NEB Buffer 2
0.5uL EcoRI
0.5uL BamHI (for parts >250bp) or XhoI (for parts <250bp)
  • Incubate at 37 on the thermocycler for 30 minutes
  • Run an analytical gel
  • Take a picture of the gel
  • Calculate the expected fragment sizes
  • Are the calculated sizes consistent with the bands on the gel?
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