Template:SBB-Protocols Enz6: Difference between revisions
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JCAnderson (talk | contribs) (New page: Set up the following 10uL reaction in a PCR tube: <pre> 6.5 uL ddH2O 2uL Miniprepped plasmid 1uL 10x NEB Buffer 2 0.5uL EcoRI 0.5uL BamHI (for parts >250bp) or XhoI (for parts <250bp) </pr...) |
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Revision as of 12:19, 2 March 2009
Set up the following 10uL reaction in a PCR tube:
6.5 uL ddH2O 2uL Miniprepped plasmid 1uL 10x NEB Buffer 2 0.5uL EcoRI 0.5uL BamHI (for parts >250bp) or XhoI (for parts <250bp)
- Incubate at 37 on the thermocycler for 30 minutes
- Run an analytical gel
- Take a picture of the gel
- Calculate the expected fragment sizes
- Are the claculated sizes consistent with the bands on the gel?
