Template:SBB-Protocols Enz6

Set up the following 10uL reaction in a PCR tube:

6.5 uL ddH2O
4uL Miniprepped plasmid
1uL 10x NEB Buffer 2
0.5uL EcoRI
0.5uL BamHI (for parts >250bp) or XhoI (for parts <250bp)

• Incubate at 37 on the thermocycler for 30 minutes
• Run an analytical gel
• Take a picture of the gel
• Calculate the expected fragment sizes
• Are the calculated sizes consistent with the bands on the gel?