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(New page: ===Picking of colonies===)
Current revision (00:18, 4 February 2009) (view source)
(Picking of colonies)
 
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===Picking of colonies===
===Picking of colonies===
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*For each construct you will pick and later miniprep 2 colonies
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*Add 4mL of LB media with the appropriate antibiotics to a clean test tube
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*Pick a well-isolated, round, and "normal" looking colony with a toothpick
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*Drop it in the test tube
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*Incubate at 37 overnight

Current revision

Picking of colonies

  • For each construct you will pick and later miniprep 2 colonies
  • Add 4mL of LB media with the appropriate antibiotics to a clean test tube
  • Pick a well-isolated, round, and "normal" looking colony with a toothpick
  • Drop it in the test tube
  • Incubate at 37 overnight
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