Template:SBB-Protocols Zymo3 - Revision history

Jonathan Kotker: /* Zymo Gel Purification */

Jonathan Kotker — Fri, 24 Feb 2012 21:09:09 GMT

Zymo Gel Purification

←Older revision		Revision as of 21:09, 24 February 2012
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	#transfer into the Zymo column inside a collection tube (small clear guys)		#transfer into the Zymo column inside a collection tube (small clear guys)
	#spin through, discard waste.		#spin through, discard waste.
-	#Add 200 uL of Zymo Wash ~~Bbuffer~~ (which is basically 70% ethanol)	+	#Add 200 uL of Zymo Wash Buffer (which is basically 70% ethanol)
	#spin through, discard waste.		#spin through, discard waste.
	#Add 200 uL of Zymo Wash Buffer		#Add 200 uL of Zymo Wash Buffer

JCAnderson — Thu, 02 Feb 2012 16:10:41 GMT

←Older revision		Revision as of 16:10, 2 February 2012
Line 8:		Line 8:
	#transfer into the Zymo column inside a collection tube (small clear guys)		#transfer into the Zymo column inside a collection tube (small clear guys)
	#spin through, discard waste.		#spin through, discard waste.
-	#Add 200 uL of A4 Wash Bbuffer (which is basically 70% ethanol)	+	#Add 200 uL of Zymo Wash Bbuffer (which is basically 70% ethanol)
	#spin through, discard waste.		#spin through, discard waste.
-	#Add 200 uL of A4 Wash Buffer	+	#Add 200 uL of Zymo Wash Buffer
	#spin through, discard waste.		#spin through, discard waste.
	#spin for 90 seconds, full speed to dry.		#spin for 90 seconds, full speed to dry.
	#elute with water into a fresh Eppendorf tube		#elute with water into a fresh Eppendorf tube

JCAnderson — Tue, 22 Feb 2011 04:14:04 GMT

←Older revision		Revision as of 04:14, 22 February 2011
Line 8:		Line 8:
	#transfer into the Zymo column inside a collection tube (small clear guys)		#transfer into the Zymo column inside a collection tube (small clear guys)
	#spin through, discard waste.		#spin through, discard waste.
-	#Add 200 uL of ~~PE buffer~~ (which is basically 70% ethanol)	+	#Add 200 uL of A4 Wash Bbuffer (which is basically 70% ethanol)
	#spin through, discard waste.		#spin through, discard waste.
-	#Add 200 uL of ~~PE buffer~~	+	#Add 200 uL of A4 Wash Buffer
	#spin through, discard waste.		#spin through, discard waste.
	#spin for 90 seconds, full speed to dry.		#spin for 90 seconds, full speed to dry.
-	#elute with ~~8.5 uL of~~ water into a fresh Eppendorf tube	+	#elute with water into a fresh Eppendorf tube

JCAnderson — Wed, 04 Feb 2009 04:55:56 GMT

Zymo Gel Purification

←Older revision		Revision as of 04:55, 4 February 2009
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	===Zymo Gel Purification===		===Zymo Gel Purification===
		+	*All spins until the drying step are 15 second full speed spins.
		+
		+	#cut out bands minimizing extra gel matter.
		+	#put in ependorf tube and add 600uL of Zymo ADB buffer (brown bottle).
		+	#heat at 55, shake and/or vortex until the gel has dissolved.
		+	#'''If the DNA is <300bp''' add 250uL of isopropanol
		+	#transfer into the Zymo column inside a collection tube (small clear guys)
		+	#spin through, discard waste.
		+	#Add 200 uL of PE buffer (which is basically 70% ethanol)
		+	#spin through, discard waste.
		+	#Add 200 uL of PE buffer
		+	#spin through, discard waste.
		+	#spin for 90 seconds, full speed to dry.
		+	#elute with 8.5 uL of water into a fresh Eppendorf tube

JCAnderson — Wed, 04 Feb 2009 04:45:13 GMT

←Older revision		Revision as of 04:45, 4 February 2009
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-	===Zymo Gel Purification ~~Cleanup~~===	+	===Zymo Gel Purification===

JCAnderson — Wed, 04 Feb 2009 04:11:41 GMT

New page: ===Zymo Gel Purification Cleanup===

New page

===Zymo Gel Purification Cleanup===